| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VANGL2 |
| Uniprot No | Q9ULK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521 aa |
| 活性数据 | MDTESQYSGYSYKSGHSRSSRKHRDRRDRHRSKSRDGGRGDKSVTIQAPGEPLLDNESTRGDERDDNWGETTTVVTGTSEHSISHDDLTRIAKDMEDSVPLDCSRHLGVAAGATLALLSFLTPLAFLLLPPLLWREELEPCGTACEGLFISVAFKLLILLLGSWALFFRRPKASLPRVFVLRALLMVLVFLLVVSYWLFYGVRILDARERSYQGVVQFAVSLVDALLFVHYLAVVLLELRQLQPQFTLKVVRSTDGASRFYNVGHLSIQRVAVWILEKYYHDFPVYNPALLNLPKSVLAKKVSGFKVYSLGEENSTNNSTGQSRAVIAAAARRRDNSHNEYYYEEAEHERRVRKRRARLVVAVEEAFTHIKRLQEEEQKNPREVMDPREAAQAIFASMARAMQKYLRTTKQQPYHTMESILQHLEFCITHDMTPKAFLERYLAAGPTIQYHKERWLAKQWTLVSEEPVTNGLKDGIVFLLKRQDFSLVVSTKKVPFFKLSEEFVDPKSHKFVMRLQSETSV |
| 分子量 | 86.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VANGL2蛋白的假设参考文献示例(仅供格式参考，非真实文献)：
---
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human VANGL2 in Planar Cell Polarity Regulation*
**作者**：Smith A, et al.
**摘要**：研究通过哺乳动物细胞系成功表达重组人VANGL2蛋白，验证其在平面细胞极性(PCP)通路中的功能，证实其与Frizzled受体互作并调控细胞定向迁移。
2. **文献名称**：*Structural Insights into the Extracellular Domain of VANGL2 via Recombinant Protein Crystallography*
**作者**：Zhang L, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度重组VANGL2胞外域，通过冷冻电镜解析其三维结构，揭示与Wnt配体结合的潜在位点及构象变化机制。
3. **文献名称**：*Recombinant VANGL2 Enhances Wnt5a Signaling in Metastatic Colorectal Cancer Models*
**作者**：Chen R, et al.
**摘要**：体外实验表明，外源性重组VANGL2蛋白通过激活非经典Wnt/PCP通路，促进结肠癌细胞侵袭能力，提示其作为癌症治疗靶标的可能性。
4. **文献名称**：*Optimization of Recombinant VANGL2 Production in E. coli for Biochemical Assays*
**作者**：Yin H, et al.
**摘要**：开发了一种高效的可溶性VANGL2重组表达及纯化方案，并应用于体外磷酸化实验，阐明其激酶调控机制。
---
如需查找真实文献，建议使用以下关键词在PubMed或Google Scholar检索：
`recombinant human VANGL2 protein expression`、`VANGL2 planar cell polarity`、`VANGL2 structural analysis`。
VANGL2 (Van Gogh-like protein 2) is a key component of the planar cell polarity (PCP) pathway, essential for regulating tissue orientation and morphogenesis during embryonic development. As a member of the VANGL family, it encodes a four-pass transmembrane protein that interacts with core PCP signaling molecules like Frizzled and Dishevelled. VANGL2 mediates cell polarity by coordinating cytoskeletal organization and directional cell movements, critical in processes such as neural tube closure, cochlear hair cell patterning, and convergent extension during gastrulation. Dysregulation of VANGL2 is linked to developmental defects (e.g., spina bifida) and cancer metastasis, attributed to its role in cell migration and tissue architecture. Recombinant human VANGL2 protein, typically produced in mammalian or insect expression systems with tags (e.g., His, Fc), retains post-translational modifications and functional epitopes. It serves as a vital tool for studying PCP mechanisms, protein-protein interactions, and screening therapeutic agents targeting developmental disorders or cancer. Recent studies also explore its involvement in ciliopathies and kidney disease, expanding its biomedical relevance.
×
