| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VAC14 |
| Uniprot No | Q08AM6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-782 aa |
| 活性数据 | MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVREFVAQNNTVQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESNKFDLVSFIPLLRERIYSNNQYARQFIISWILVLESVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDELDELRPGQRQAEPTPDDALPKQEGTASGGPDGSCDSSFSSGISVFTAASTERAPVTLHLDGIVQVLNCHLSDTAIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQTDDPGPLDGPDLQASHSELQVPTPGRAGLLNTSGTKGLECSPSTPTMNSYFYKFMINLLKRFSSERKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDSLKAAPKSQKADSPSIDYAELLQHFEKVQNKHLEVRHQRSGRGDHLDRRVVL |
| 分子量 | 114.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人VAC14蛋白的3篇参考文献，涵盖其功能、相互作用及结构研究：
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1. **文献名称**：*"The Vac14-interaction network regulates PI(3.5)P2 synthesis and autophagy"*
**作者**：Sbrissa D, et al.
**摘要**：该研究通过重组表达人源VAC14蛋白，揭示了其与PIKfyve和Fig4形成的复合体对PI(3.5)P2脂质合成的调控作用，并发现VAC14缺失会导致细胞内溶酶体功能紊乱及自噬异常。
2. **文献名称**：*"VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3.5)P2 in yeast and mouse cells"*
**作者**：Hasegawa J, et al.
**摘要**：研究利用重组人VAC14蛋白，在哺乳动物细胞中验证了其在PI3P向PI(3.5)P2快速转化中的核心作用，并发现该复合体对溶酶体形态和内吞过程至关重要。
3. **文献名称**：*"Structural insights into the assembly of the human VAC14-adaptor complex"*
**作者**：Dong S, et al.
**摘要**：通过重组表达和纯化人VAC14蛋白，结合冷冻电镜技术解析了其与PIKfyve和Fig4的三元复合体结构，揭示了VAC14在稳定复合体构象中的关键结构域及作用机制。
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以上文献均涉及重组人VAC14蛋白的功能或结构研究，可为其在信号通路中的作用提供参考。如需扩展，可关注其与神经退行性疾病关联的研究。
VAC14. a conserved metazoan protein, plays a critical role in regulating intracellular membrane dynamics and phosphoinositide signaling. It functions as a scaffold component of the FAB1/PIKFYVE complex, which governs the synthesis and turnover of phosphatidylinositol-3.5-bisphosphate [PI(3.5)P₂], a key lipid messenger involved in endolysosomal trafficking, autophagy, and osmotic stress response. VAC14 interacts with FIG4 (a PI(3.5)P₂ phosphatase) and PIKFYVE (a lipid kinase) to maintain PI(3.5)P₂ homeostasis, thereby influencing vesicle maturation, lysosomal function, and organelle integrity. Dysregulation of VAC14 is linked to neurological disorders, including hereditary spastic paraplegia and Yunis-Varon syndrome, as well as peripheral neuropathy in animal models.
Recombinant human VAC14 protein, typically produced in bacterial or mammalian expression systems, enables biochemical and structural studies to dissect its role in complex assembly, lipid modulation, and disease-associated mutations. Its purified form facilitates in vitro binding assays, enzyme activity analyses, and drug screening for conditions tied to PI(3.5)P₂ imbalance. Recent studies also highlight VAC14’s potential crosstalk with mTOR signaling and inflammation pathways, expanding its relevance in cancer and neurodegenerative research. Despite progress, mechanistic details of VAC14’s regulatory interplay within the FAB1/PIKFYVE complex remain under investigation, underscoring the importance of recombinant protein tools in advancing this field.
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