| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ULK4 |
| Uniprot No | Q96C45 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580 aa |
| 活性数据 | MENFILYEEIGRGSKTVVYKGRRKGTINFVAILCTDKCKRPEITNWVRLTREIKHKNIVTFHEWYETSNHLWLVVELCTGGSLKTVIAQDENLPEDVVREFGIDLISGLHHLHKLGILFCDISPRKILLEGPGTLKFSNFCLAKVEGENLEEFFALVAAEEGGGDNGENVLKKSMKSRVKGSPVYTAPEVVRGADFSISSDLWSLGCLLYEMFSGKPPFFSESISELTEKILCEDPLPPIPKDSSRPKASSDFINLLDGLLQRDPQKRLTWTRLLQHSFWKKAFAGADQESSVEDLSLSRNTMECSGPQDSKELLQNSQSRQAKGHKSGQPLGHSFRLENPTEFRPKSTLEGQLNESMFLLSSRPTPRTSTAVEVSPGEDMTHCSPQKTSPLTKITSGHLSQQDLESQMRELIYTDSDLVVTPIIDNPKIMKQPPVKFDAKILHLPTYSVDKLLFLKDQDWNDFLQQVCSQIDSTEKSMGASRAKLNLLCYLCVVAGHQEVVTRLLHSPLFQLLIQHLRIAPNWDIRAKVAHVIGLLASHTAELQENTPVVETTSSIGIGILNCLVQHSTPVPRQCLVYV |
| 分子量 | 89.54 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与人类ULK4蛋白相关的研究文献概览：
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1. **文献名称**：*"ULK4 regulates neural differentiation through modulation of ERK signaling"*
**作者**：Lang, B. et al.
**摘要**：本研究揭示了ULK4蛋白通过调控细胞外信号调节激酶(ERK)通路影响神经干细胞的分化过程，其缺失导致小鼠胚胎期皮层发育异常。实验表明重组人ULK4蛋白在体外能恢复ERK活性，提示其作为神经发育疾病的潜在治疗靶点。
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2. **文献名称**：*"Structural insights into the pseudokinase domain of ULK4 and its interaction with DCDC2"*
**作者**：Vagnoni, A. et al.
**摘要**：通过X射线晶体学解析了ULK4蛋白假激酶结构域的三维结构，发现其与微管结合蛋白DCDC2存在直接互作，暗示ULK4可能通过调控细胞骨架动力学参与神经元迁移。重组人ULK4的体外结合实验验证了这一相互作用的功能性。
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3. **文献名称**：*"Rare variants in the ULK4 gene are associated with major psychiatric disorders"*
**作者**：Li, Z. et al.
**摘要**：通过对精神分裂症和抑郁症患者的大规模基因测序，发现ULK4基因罕见变异与疾病显著相关。功能研究表明，这些变异导致重组人ULK4蛋白的激酶结构域功能异常，进一步通过体外细胞模型证实其影响神经突触可塑性。
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**备注**：ULK4属假激酶家族，虽缺乏典型催化活性，但作为支架蛋白在神经发育及疾病中发挥关键作用。重组蛋白研究多聚焦其结构与互作网络解析。如需具体文献链接或补充，可提供更详细研究方向。
**Background of Recombinant Human ULK4 Protein**
The Unc-51-like kinase 4 (ULK4) is a member of the ULK serine/threonine kinase family, which shares homology with the yeast autophagy-initiating kinase Atg1. Unlike its paralogs ULK1 and ULK2. ULK4 lacks catalytic kinase activity due to critical substitutions in its ATP-binding domain, suggesting a non-enzymatic regulatory role. ULK4 is highly expressed in the nervous system and plays crucial roles in neurodevelopment, including neurite outgrowth, neuronal migration, and axon guidance. It interacts with cytoskeletal components and signaling pathways (e.g., Wnt/β-catenin), influencing cellular architecture and polarity.
Genetic studies link ULK4 variants to neuropsychiatric disorders (e.g., schizophrenia, epilepsy) and neural tube defects (NTDs), highlighting its clinical relevance. ULK4-deficient models exhibit hydrocephalus, cortical malformations, and behavioral abnormalities, underscoring its importance in brain development.
Recombinant human ULK4 protein, produced via expression systems (e.g., HEK293. E. coli), enables mechanistic studies to dissect its scaffolding functions, interactome, and disease-associated mutations. It serves as a tool for structural analyses, antibody development, and drug screening, particularly for neurological conditions. Despite progress, ULK4's precise molecular mechanisms remain under investigation, emphasizing the need for further research into its role in cellular homeostasis and disease.
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