| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBA6 |
| Uniprot No | Q9BQE3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-449 aa |
| 活性数据 | MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY |
| 分子量 | 75.13 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBA6蛋白的3篇模拟参考文献示例(注：内容为假设性概括，非真实文献)：
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1. **文献名称**: *Structural insights into recombinant human TUBA6 and its role in microtubule assembly*
**作者**: Smith J., et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人TUBA6蛋白，通过X射线晶体学解析其三维结构，揭示了TUBA6与其他α-微管蛋白亚型的结构差异，并发现其C端结构域在微管动态组装中的调控作用。
2. **文献名称**: *TUBA6 mutations disrupt neuronal migration via altered microtubule dynamics*
**作者**: Al-Mazroea S., et al.
**摘要**: 通过全外显子测序发现TUBA6基因突变与儿童神经发育障碍相关。体外实验表明，突变型重组TUBA6蛋白导致微管稳定性下降，影响神经元突触形成和小鼠皮层神经元迁移，提示TUBA6在神经发育中的关键作用。
3. **文献名称**: *High-yield production of recombinant TUBA6 for functional screening of microtubule-targeting agents*
**作者**: Braun L., et al.
**摘要**: 开发了一种昆虫细胞表达系统高效制备重组人TUBA6蛋白的方法，优化后的蛋白纯度>95%。该蛋白被用于高通量药物筛选，鉴定出多个新型微管稳定剂，为抗癌药物开发提供工具。
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(注：以上文献为示例性内容，实际研究中请通过学术数据库核实具体文献。)
**Background of Recombinant Human TUBA6 Protein**
Tubulin alpha-6 (TUBA6), a member of the α-tubulin family, is a critical component of microtubules—dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintenance of cell shape. α-tubulin monomers polymerize with β-tubulin to form heterodimers, the building blocks of microtubules. TUBA6. encoded by the *TUBA6* gene, is one of several α-tubulin isotypes, each exhibiting tissue-specific expression or functional specialization. While many α-tubulin isoforms are well-characterized, TUBA6 remains less studied, though it is implicated in developmental processes and neuronal function.
Recombinant TUBA6 protein is produced via genetic engineering, typically using bacterial or mammalian expression systems, enabling controlled study of its biochemical properties and interactions. This recombinant form retains conserved structural features, including GTP-binding domains critical for microtubule assembly and stability. Research applications include elucidating TUBA6-specific roles in microtubule dynamics, understanding its potential dysregulation in diseases (e.g., neurodevelopmental disorders or cancer), and screening microtubule-targeting drugs. Its recombinant availability also aids in structural studies, such as cryo-EM, to resolve mechanisms underlying tubulin isotype diversity. Overall, TUBA6 serves as a valuable tool for probing microtubule-related cellular processes and developing therapeutic strategies.
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