| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBA3D |
| Uniprot No | P0DPH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450 aa |
| 活性数据 | MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKYDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGVDSVEAEAEEGEEY |
| 分子量 | 76.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TUBA3D蛋白的参考文献及摘要概括，均为示例性内容(实际文献可能需要根据最新数据库检索)：
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1. **文献名称**：*"Characterization of recombinant human TUBA3D and its role in microtubule dynamics"*
**作者**：Zhang L, et al.
**摘要**：本研究首次在大肠杆菌中成功表达并纯化了重组人TUBA3D蛋白，分析了其体外微管聚合能力及对紫杉醇药物敏感性的影响，揭示了TUBA3D在肿瘤细胞有丝分裂中的潜在作用。
2. **文献名称**：*"TUBA3D mutations disrupt neuronal migration and cortical development"*
**作者**：Smith J, et al.
**摘要**：通过CRISPR技术构建TUBA3D突变体，发现其异常表达会干扰小鼠皮层神经元的迁移，提示TUBA3D在脑发育中的关键功能及与神经发育障碍的可能关联。
3. **文献名称**：*"Proteomic analysis of TUBA3D-interacting proteins in glioblastoma cells"*
**作者**：Chen H, et al.
**摘要**：利用重组TUBA3D蛋白进行免疫共沉淀实验，鉴定出其在胶质母细胞瘤中与MAP2和动力蛋白(Dynein)的相互作用，为癌症靶向治疗提供新机制。
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**注**：以上为示例，实际文献需通过PubMed、Google Scholar等平台以"TUBA3D"或"tubulin alpha 3D"为关键词检索近5-10年研究。若研究较少，可扩展至β-微管蛋白家族或重组微管蛋白技术的相关文献。
**Background of Recombinant Human TUBA3D Protein**
TUBA3D, a member of the α-tubulin family, is one of the critical isoforms involved in microtubule formation. Microtubules, dynamic cytoskeletal polymers composed of α/β-tubulin heterodimers, play essential roles in cell division, intracellular transport, and maintenance of cell shape. TUBA3D is encoded by the *TUBA3D* gene and exhibits tissue-specific expression patterns, particularly enriched in testis and brain tissues, suggesting specialized roles in these organs. Unlike ubiquitously expressed α-tubulin isoforms (e.g., TUBA1A/B), TUBA3D’s regulation and function remain less characterized, though it may contribute to microtubule diversity and functional adaptation in specific cellular contexts.
Recombinant TUBA3D protein is typically produced using *in vitro* expression systems (e.g., *E. coli* or mammalian cells) for biochemical and structural studies. Its recombinant form enables investigations into isoform-specific interactions with microtubule-associated proteins (MAPs), motor proteins, or anticancer drugs (e.g., taxanes). Dysregulation of TUBA3D has been implicated in neurodevelopmental disorders and cancer, where altered microtubule dynamics may disrupt mitosis or neuronal migration. As a research tool, recombinant TUBA3D aids in deciphering the molecular basis of microtubule-related pathologies and screening isoform-targeted therapeutics, bridging gaps in understanding tubulin isoform specificity in health and disease.
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