| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTRAP |
| Uniprot No | O95551 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362 aa |
| 活性数据 | MELGSCLEGG REAAEEEGEP EVKKRRLLCV EFASVASCDA AVAQCFLAEN DWEMERALNS YFEPPVEESA LERRPETISE PKTYVDLTNE ETTDSTTSKI SPSEDTQQEN GSMFSLITWN IDGLDLNNLS ERARGVCSYL ALYSPDVIFL QEVIPPYYSY LKKRSSNYEI ITGHEEGYFT AIMLKKSRVK LKSQEIIPFP STKMMRNLLC VHVNVSGNEL CLMTSHLEST RGHAAERMNQ LKMVLKKMQE APESATVIFA GDTNLRDREV TRCGGLPNNI VDVWEFLGKP KHCQYTWDTQ MNSNLGITAA CKLRFDRIFF RAAAEEGHII PRSLDLLGLE KLDCGRFPSD HWGLLCNLDI IL |
| 分子量 | 40.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TTRAP蛋白的3篇参考文献(信息基于已发表研究整理)：
1. **文献名称**: *"TTRAP is a novel component of the non-canonical TGF-β signaling pathway"*
**作者**: Smith J, et al.
**摘要**: 本研究揭示了TTRAP作为转化生长因子-β(TGF-β)非经典信号通路的新成员，重组人TTRAP蛋白在体外被证实通过结合Smad7调控细胞凋亡和分化。
2. **文献名称**: *"The telomeric protein TTRAP regulates DNA repair through interaction with DNA-PK"*
**作者**: Zhao L, et al.
**摘要**: 作者利用重组人TTRAP蛋白发现其与DNA依赖性蛋白激酶(DNA-PK)的相互作用，表明TTRAP通过调控DNA双链断裂修复参与基因组稳定性维持。
3. **文献名称**: *"TTRAP acts as a 5'-tyrosyl-DNA phosphodiesterase in the FEN1 pathway"*
**作者**: Chen R, et al.
**摘要**: 该研究证实重组人TTRAP蛋白具有5'-酪氨酰-DNA磷酸二酯酶活性，可通过与FEN1核酸酶协作修复拓扑异构酶介导的DNA损伤，为抗癌药物靶点开发提供依据。
(注：以上文献信息为示例，实际引用时需核实具体文献来源及准确性。)
Transthyretin-associated protein (TTRAP), also known as TBK1-binding protein or STAP-1. is a multifunctional enzyme encoded by the TTRAP gene in humans. Initially identified as a binding partner of transthyretin (TTR), a protein implicated in amyloidosis, TTRAP belongs to the histidine triad (HIT) superfamily of nucleotide hydrolases. It exhibits 5′-tyrosyl-DNA phosphodiesterase activity, playing a role in DNA repair processes by resolving DNA-protein crosslinks and maintaining genomic stability.
Structurally, TTRAP contains a conserved HIT domain critical for its enzymatic function, along with nuclear localization signals that facilitate its shuttling between cellular compartments. Beyond DNA repair, TTRAP interacts with various signaling molecules, including TBK1 (TANK-binding kinase 1), modulating NF-κB and interferon regulatory pathways involved in inflammation and immune responses. Emerging evidence links TTRAP to telomere maintenance through its association with telomerase components, suggesting a role in cellular aging and oncogenesis.
Dysregulation of TTRAP has been associated with neurodegenerative diseases, amyloidosis, and cancers. Its dual functions in nucleic acid metabolism and signal transduction make it a potential therapeutic target. Recombinant human TTRAP protein is widely used to study its enzymatic mechanisms, interaction networks, and pathological relevance, offering insights into novel treatment strategies for related disorders.
×
