| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBA3E |
| Uniprot No | Q6PEY2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450 aa |
| 活性数据 | MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVVDEVRT GTYRQLFHPE QLITGKEDAA SNYARGHYTI GKEIVDLVLD RIRKLADLCT GLQGFLIFHS FGGGTGSGFA SLLMERLSVD YSKKSKLEFA IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCMLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LVHKFDLMYA KWAFVHWYVG EGMEEGEFSE AREDLAALEK DCEEVGVDSV EAEAEEGEAY |
| 分子量 | 49.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是三篇关于重组人TUBA3E蛋白的模拟参考文献(仅供示例参考，非真实文献)：
1. **文献名称**：*"Recombinant expression and functional characterization of human TUBA3E in microtubule dynamics"*
**作者**：Zhang, L. et al.
**摘要**：研究报道了TUBA3E蛋白在大肠杆菌中的重组表达与纯化，分析了其体外微管聚合动力学特性，揭示了该异构体在细胞分裂中可能具有独特的微管稳定性调控作用。
2. **文献名称**：*"TUBA3E overexpression correlates with chemoresistance in colorectal cancer"*
**作者**：Kim, J.H. & Patel, S.
**摘要**：通过免疫组织化学和体外实验证明，TUBA3E在结直肠癌细胞中高表达并与紫杉醇耐药性相关，重组蛋白的靶向干预可部分逆转耐药表型。
3. **文献名称**：*"Structural insights into TUBA3E isotype-specific interactions with kinesin motors"*
**作者**：Watanabe, T. et al.
**摘要**：利用重组TUBA3E蛋白的冷冻电镜结构解析，发现其C端结构域与驱动蛋白KIF5B的结合模式不同于其他α-微管蛋白异构体，提示其在神经轴突运输中的特殊功能。
**注**：以上文献为模拟生成，实际研究中建议通过学术数据库(如PubMed、Web of Science)以“TUBA3E”、“tubulin alpha 3e”等关键词检索最新成果。
Tubulin alpha-3E (TUBA3E) is a member of the α-tubulin protein family, which plays a fundamental role in cellular structure, motility, and intracellular transport. As a core component of microtubules, TUBA3E contributes to dynamic cytoskeletal networks critical for cell division, organelle positioning, and maintenance of cell shape. Unlike ubiquitously expressed α-tubulin isoforms, TUBA3E exhibits tissue-specific expression patterns, with notable enrichment in the testes, suggesting a specialized role in germ cell division or sperm development.
Recombinant TUBA3E protein is produced using biotechnological methods, such as expression in bacterial or mammalian systems followed by purification via affinity chromatography. This engineered protein retains the ability to polymerize into microtubules when combined with β-tubulin, enabling functional studies on microtubule dynamics. Its recombinant form is particularly valuable for investigating isoform-specific contributions to cellular processes, as slight sequence variations among α-tubulins can influence microtubule stability, interaction with motor proteins, or responsiveness to pharmacological agents like taxanes or colchicine.
Current research focuses on TUBA3E’s potential implications in diseases, including cancer (where tubulin dysregulation affects mitosis) and neurodegenerative disorders. It also serves as a tool for screening microtubule-targeting drugs or understanding mechanisms of drug resistance. Ongoing studies aim to unravel how its tissue-specific expression fine-tunes microtubule behavior in specialized cellular contexts.
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