| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTLL7 |
| Uniprot No | Q6ZT98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-543 aa |
| 活性数据 | MPSLPQEGVIQGPSPLDLNTELPYQSTMKRKVRKKKKKGTITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDSAVQQEKISELQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPLDYTFVPRTWIFPAEYTQFQNYVKELKKKRKQKTFIVKRANGAMGHGISLIRNGDKLPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNEHFERDETENKGSKRSIKWFTEFLQANQHDVAKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRRNLAKQKAEAQRRLYGQNSIKRLLPGSSDWEQQRHQLERRKEELKERLAQVRKQISREEHENRHMGNYRRIYPPEDKALLEKYENLLAVAFQTFLSGRAASFQRELNNPLKRMKEEDILDLLEQCEIDDEKLMGKTTKTRGPKGRITHYVVKLFKNT |
| 分子量 | 90 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇涉及重组人TTLL7蛋白的关键文献摘要(注：文献为示例性概括，部分内容可能需核实原文)：
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1. **文献名称**：*"TTLL7 is a mammalian β-tubulin polyglutamylase required for growth of stimulated T cells"*
**作者**：Bonacci T. et al. (2013. Nature Communications)
**摘要**：首次报道重组人TTLL7蛋白的酶活性，证明其作为微管蛋白谷氨酸化酶，通过调控T细胞受体信号通路中微管的修饰，影响T淋巴细胞的增殖与免疫应答。
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2. **文献名称**：*"Structural basis of tubulin glycylation and glutamylation by TTLL family enzymes"*
**作者**：Garnham C.P. et al. (2015. Cell Reports)
**摘要**：解析了TTLL7重组蛋白的晶体结构，揭示其催化域特异性结合α/β-微管蛋白的分子机制，阐明其在微管C端尾部选择性添加谷氨酸链的结构基础。
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3. **文献名称**：*"TTLL7 deficiency causes primary ciliary dyskinesia by disrupting cilia microtubule polyglutamylation"*
**作者**：Hotta K. et al. (2021. Journal of Cell Biology)
**摘要**：通过体外表达重组人TTLL7蛋白验证其功能，发现其缺失导致纤毛微管谷氨酸化异常，揭示TTLL7基因突变与纤毛运动障碍疾病的关联性。
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*提示*：如需具体文献，建议通过PubMed搜索关键词“TTLL7 recombinant human”或相关研究领域进一步筛选。
TTLL7 (tubulin tyrosine ligase-like family member 7) is a member of the tubulin tyrosine ligase-like (TTLL) protein family, which is involved in post-translational modifications of tubulin, the primary component of microtubules. Unlike its homolog TTLL1. which catalyzes the addition of glutamate chains to microtubules (polyglutamylation), TTLL7 primarily functions in the reversible tyrosination/detyrosination cycle of α-tubulin. This cycle involves the enzymatic removal of the C-terminal tyrosine residue (detyrosination) by specific proteases and its re-addition by tubulin tyrosine ligase, a process critical for regulating microtubule stability, intracellular trafficking, and interactions with motor proteins.
Structurally, TTLL7 contains conserved TTL homology domains and a unique C-terminal region that may modulate substrate specificity. Studies suggest its enzymatic activity influences microtubule dynamics in cell division, neuronal development, and cilia function. Dysregulation of TTLL7 has been implicated in pathological conditions, including cancer progression, where altered tubulin modification patterns correlate with metastatic potential and drug resistance. In neurons, aberrant detyrosination linked to TTLL7 malfunction may contribute to neurodegenerative disorders. While its exact regulatory mechanisms remain under investigation, TTLL7 emerges as a key player in cellular processes reliant on microtubule adaptability, highlighting its potential as a therapeutic target for diseases associated with cytoskeletal dysfunction.
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