| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPYL1 |
| Uniprot No | Q9H0U9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437 aa |
| 活性数据 | MSGLDGVKRT TPLQTHSIII SDQVPSDQDA HQYLRLRDQS EATQVMAEPG EGGSETVALP PPPPSEEGGV PQDAAGRGGT PQIRVVGGRG HVAIKAGQEE GQPPAEGLAA ASVVMAADRS LKKGVQGGEK ALEICGAQRS ASELTAGAEA EAEEVKTGKC ATVSAAVAER ESAEVVKEGL AEKEVMEEQM EVEEQPPEGE EIEVAEEDRL EEEAREEEGP WPLHEALRMD PLEAIQLELD TVNAQADRAF QQLEHKFGRM RRHYLERRNY IIQNIPGFWM TAFRNHPQLS AMIRGQDAEM LRYITNLEVK ELRHPRTGCK FKFFFRRNPY FRNKLIVKEY EVRSSGRVVS LSTPIIWRRG HEPQSFIRRN QDLICSFFTW FSDHSLPESD KIAEIIKEDL WPNPLQYYLL REGVRRARRR PLREPVEIPR PFGFQSG |
| 分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
**1. 文献名称**: *TSPYL1 is a novel tumor suppressor gene in hepatocellular carcinoma*
**作者**: Nagai H. et al.
**摘要**: 该研究揭示TSPYL1在肝癌组织中显著低表达，过表达可抑制细胞增殖并诱导细胞周期停滞及凋亡。机制涉及与p53的互作增强下游靶基因转录，确立其作为新型抑癌因子。
**2. 文献名称**: *Epigenetic silencing of TSPYL1 contributes to cervical cancer progression through HDAC-mediated chromatin remodeling*
**作者**: Li Y. et al.
**摘要**: 研究发现宫颈癌中TSPYL1因启动子高甲基化而沉默，恢复表达可抑制肿瘤生长并增强化疗敏感性。其机制与抑制HDAC活性及调节染色质可及性相关。
**3. 文献名称**: *TSPYL1 regulates SIRT1 activity through direct binding and modulation of histone H3 lysine 9 acetylation*
**作者**: Kim J.W. et al.
**摘要**: 通过重组蛋白实验证明，TSPYL1通过结合组蛋白H3K9乙酰化区域增强SIRT1的去乙酰化酶活性，揭示其在表观遗传调控及代谢应激响应中的关键作用。
**4. 文献名称**: *Loss of TSPYL1 disrupts the cell cycle and sensitizes ovarian cancer cells to cisplatin therapy*
**作者**: Chen L. et al.
**摘要**: 在卵巢癌中，TSPYL1缺失导致G1/S期阻滞并促进细胞对顺铂的敏感性。重组TSPYL1蛋白可恢复周期蛋白依赖激酶抑制剂p21的表达，提示其作为化疗增敏靶点潜力。
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**说明**：文献均为虚拟构建，实际研究中需通过PubMed/Google Scholar检索真实研究(关键词：TSPYL1、recombinant protein、cancer mechanism)。
**Background of Recombinant Human TSPYL1 Protein**
TSPYL1 (Testis-Specific Protein Y-Encoded-Like 1), also known as TSPY-Like 1. belongs to the TSPY/SET/NAP1L superfamily, which is involved in chromatin remodeling and transcriptional regulation. This nucleoprotein is ubiquitously expressed in human tissues, particularly in the nucleus, where it interacts with chromatin and modulates gene expression. Structurally, TSPYL1 contains a conserved nucleosome assembly protein (NAP) domain, enabling its role in maintaining chromatin structure and epigenetic regulation.
Functionally, TSPYL1 acts as a tumor suppressor by regulating cell cycle progression, apoptosis, and DNA damage response. It interacts with critical regulatory proteins, such as p53 and histone deacetylases (HDACs), to influence pathways linked to cancer development and neurodegenerative disorders. Mutations or dysregulation of TSPYL1 have been associated with various cancers, including neuroblastoma, ovarian cancer, and glioblastoma, as well as neurological conditions like sudden infant death syndrome (SIDS).
Recombinant human TSPYL1 protein, produced via *in vitro* expression systems, serves as a vital tool for studying its molecular mechanisms, structural interactions, and therapeutic potential. Its applications span cancer research, drug discovery, and investigations into epigenetic disorders, offering insights into targeted therapies and diagnostic biomarkers. Ongoing studies continue to explore its role in cellular homeostasis and disease pathogenesis.
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