| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM55 |
| Uniprot No | Q9BYV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548 aa |
| 活性数据 | MSASLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP YLPTRGGTTM ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKSD QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAILV GSNDRVQGVI SQLEDTCKTI EECCRKQKQE LCEKFDYLYG ILEERKNEMT QVITRTQEEK LEHVRALIKK YSDHLENVSK LVESGIQFMD EPEMAVFLQN AKTLLKKISE ASKAFQMEKI EHGYENMNHF TVNLNREEKI IREIDFYRED EDEEEEEGGE GEKEGEGEVG GEAVEVEEVE NVQTEFPGED ENPEKASELS QVELQAAPGA LPVSSPEPPP ALPPAADAPV TQGEVVPTGS EQTTESETPV PAAAETADPL FYPSWYKGQT RKATTNPPCT PGSEGLGQIG PPGSEDSNVR KAEVAAAAAS ERAAVSGKET SAPAATSQIG FEAPPLQGQA AAPASGSGAD SEPARHIFSF SWLNSLNE |
| 分子量 | 60.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM55蛋白的3篇代表性文献示例(内容基于实际研究方向，文献为假设性概括)：
1. **文献名称**：*TRIM55 regulates ubiquitination and turnover of myogenic factors in skeletal muscle differentiation*
**作者**：Chen L, Wang Y, et al.
**摘要**：研究了TRIM55在骨骼肌分化中的调控作用，发现其通过泛素化途径降解抑制肌生成的转录因子，促进肌细胞分化。重组TRIM55蛋白的体外实验证实其E3泛素连接酶活性。
2. **文献名称**：*TRIM55 deficiency exacerbates cardiac hypertrophy via modulating NF-κB signaling*
**作者**：Tanaka K, Suzuki H, et al.
**摘要**：在小鼠模型中验证了TRIM55通过调控NF-κB信号通路抑制病理性心肌肥大。利用重组TRIM55蛋白干预实验表明其具有潜在的心脏保护功能。
3. **文献名称**：*Expression and purification of recombinant human TRIM55 for functional analysis of its RING domain*
**作者**：Zhang R, Liu M, et al.
**摘要**：报道了重组人TRIM55蛋白在大肠杆菌中的高效表达与纯化方法，并验证其RING结构域对泛素化反应的关键作用，为后续机制研究提供工具。
**备注**：上述文献内容为领域内典型研究方向(如肌肉分化、疾病机制、蛋白重组技术)的概括性示例，具体文献需通过学术数据库检索确认。
TRIM55. also known as muscle RING-finger protein 3 (MuRF3), is a member of the tripartite motif (TRIM) protein family, characterized by a conserved RING domain, B-box motifs, and a coiled-coil region. Predominantly expressed in skeletal and cardiac muscles, TRIM55 plays critical roles in muscle development, maintenance, and stress response. It is implicated in ubiquitination processes, regulating protein turnover via the ubiquitin-proteasome system, particularly in muscle atrophy and sarcomere organization.
Studies highlight its interaction with structural proteins like titin and its involvement in signaling pathways related to muscle hypertrophy or degradation. For instance, TRIM55 may antagonize atrophy by modulating ubiquitin ligase activity or stabilizing cytoskeletal components. Dysregulation of TRIM55 has been linked to muscular dystrophies and cardiomyopathies, underscoring its importance in muscle integrity.
Emerging research also explores its non-muscle roles, including potential involvement in cancer and inflammation, though mechanistic insights remain limited. Targeting TRIM55 could offer therapeutic avenues for muscle-wasting disorders or cardiac dysfunction, but further studies are needed to elucidate its precise molecular interactions and regulatory networks. Overall, TRIM55 represents a key regulator of muscle homeostasis with broader implications in human diseases.
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