| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM10 |
| Uniprot No | Q9UDY6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481 aa |
| 活性数据 | MASAASVTSL ADEVNCPICQ GTLREPVTID CGHNFCRACL TRYCEIPGPD LEESPTCPLC KEPFRPGSFR PNWQLANVVE NIERLQLVST LGLGEEDVCQ EHGEKIYFFC EDDEMQLCVV CREAGEHATH TMRFLEDAAA PYREQIHKCL KCLRKEREEI QEIQSRENKR MQVLLTQVST KRQQVISEFA HLRKFLEEQQ SILLAQLESQ DGDILRQRDE FDLLVAGEIC RFSALIEELE EKNERPAREL LTDIRSTLIR CETRKCRKPV AVSPELGQRI RDFPQQALPL QREMKMFLEK LCFELDYEPA HISLDPQTSH PKLLLSEDHQ RAQFSYKWQN SPDNPQRFDR ATCVLAHTGI TGGRHTWVVS IDLAHGGSCT VGVVSEDVQR KGELRLRPEE GVWAVRLAWG FVSALGSFPT RLTLKEQPRQ VRVSLDYEVG WVTFTNAVTR EPIYTFTASF TRKVIPFFGL WGRGSSFSLS S |
| 分子量 | 55.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TRIM10蛋白的3-4篇参考文献的简要总结(*注：以下为模拟示例，具体文献可能需要根据实际数据库检索验证*)：
1. **文献名称**：*"Recombinant human TRIM10 protein modulates erythroid differentiation via ubiquitination pathways"*
**作者**：Chen X, Li R, Wang J.
**摘要**：研究通过HEK293细胞表达重组TRIM10蛋白，发现其通过泛素化修饰调控GATA-1转录因子，影响红细胞分化和成熟。
2. **文献名称**：*"Structural and functional characterization of the TRIM10 B-box domain in antiviral immunity"*
**作者**：Suzuki M, et al.
**摘要**：解析了重组TRIM10蛋白的B-box结构域晶体结构，证明其通过介导蛋白互作抑制RNA病毒复制，并增强I型干扰素信号通路。
3. **文献名称**：*"TRIM10 as a novel regulator in NF-κB signaling: Insights from recombinant protein studies"*
**作者**：Gupta S, et al.
**摘要**：利用纯化的重组TRIM10蛋白，发现其与IKKγ相互作用促进NF-κB活化，提示其在炎症和癌症中的潜在作用。
4. **文献名称**：*"Development of a high-yield recombinant TRIM10 expression system for biochemical assays"*
**作者**：Kim T, Park H.
**摘要**：优化了昆虫杆状病毒系统中TRIM10的表达和纯化工艺，获得高活性蛋白，用于后续泛素连接酶活性及底物筛选实验。
(**注意**：以上内容为基于TRIM蛋白家族常见研究方向的模拟生成，实际文献需通过学术数据库检索确认。)
TRIM10. a member of the tripartite motif (TRIM) protein family, is characterized by its conserved RING finger, B-box, and coiled-coil domains. It belongs to the TRIM10/21/39 subfamily and is encoded in the major histocompatibility complex (MHC) class I region on human chromosome 6. TRIM10 is predominantly expressed in hematopoietic cells, particularly erythroid precursors, and plays a regulatory role in innate immunity, erythrocyte differentiation, and cellular stress responses. Its RING domain confers E3 ubiquitin ligase activity, enabling post-translational modification of target proteins via ubiquitination, which influences protein stability, signaling pathways, or subcellular localization.
Studies suggest TRIM10 interacts with intermediates in erythropoiesis, such as band 3 anion transport protein, to regulate red blood cell maturation. Additionally, it may modulate antiviral defenses by targeting viral components or host factors involved in immune signaling. Dysregulation of TRIM10 has been linked to pathological conditions, including hemolytic anemias, viral infections, and certain cancers. Recombinant human TRIM10 protein, produced via expression systems like mammalian or bacterial cells, serves as a vital tool for elucidating its molecular mechanisms, substrate identification, and therapeutic exploration. Its structural and functional versatility underscores its relevance in hematology, immunology, and disease research.
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