| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIB1 |
| Uniprot No | Q96RU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372 aa |
| 活性数据 | MRVGPVRSAM SGASQPRGPA LLFPATRGVP AKRLLDADDA AAVAAKCPRL SECSSPPDYL SPPGSPCSPQ PPPAAPGAGG GSGSAPGPSR IADYLLLPLA EREHVSRALC IHTGRELRCK VFPIKHYQDK IRPYIQLPSH SNITGIVEVI LGETKAYVFF EKDFGDMHSY VRSRKRLREE EAARLFKQIV SAVAHCHQSA IVLGDLKLRK FVFSTEERTQ LRLESLEDTH IMKGEDDALS DKHGCPAYVS PEILNTTGTY SGKAADVWSL GVMLYTLLVG RYPFHDSDPS ALFSKIRRGQ FCIPEHISPK ARCLIRSLLR REPSERLTAP EILLHPWFES VLEPGYIDSE IGTSDQIVPE YQEDSDISSF FC |
| 分子量 | 41.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人源TRIB1蛋白的3-4篇参考文献概览：
1. **文献名称**：*Tribbles pseudokinase 1 (TRIB1) regulates MCL1 protein stability through interaction with USP7 and USP9X*
**作者**：Yamamoto Y., et al.
**摘要**：该研究揭示了TRIB1通过与去泛素化酶USP7和USP9X结合，调控抗凋亡蛋白MCL1的稳定性，影响肿瘤细胞的存活和对化疗的敏感性。
2. **文献名称**：*TRIB1 regulates acute myeloid leukemia progression via ubiquitination of C/EBPα*
**作者**：Hassan S., et al.
**摘要**：作者发现TRIB1通过促进转录因子C/EBPα的泛素化降解，干扰造血分化，从而在急性髓系白血病(AML)发生中发挥致癌作用。
3. **文献名称**：*TRIB1 modulates lipid metabolism via hepatic APOE/ERAD-dependent regulation*
**作者**：Sasaki A., et al.
**摘要**：研究表明，TRIB1通过调节肝脏中载脂蛋白E(APOE)的内质网相关降解(ERAD)通路，影响脂质代谢及动脉粥样硬化的发展。
4. **文献名称**：*Structural basis of TRIB1 interaction with COP1 to modulate C/EBPα degradation*
**作者**：Yoshida M., et al.
**摘要**：通过解析TRIB1的晶体结构，揭示了其N端结构域与E3泛素连接酶COP1的相互作用机制，阐明了TRIB1促进C/EBPα降解的结构基础。
上述文献涉及TRIB1在癌症、代谢疾病中的作用及其分子机制，涵盖功能、调控和结构研究。
Tribbles homolog 1 (TRIB1) is a pseudokinase protein encoded by the *TRIB1* gene in humans, belonging to the conserved Tribbles family. Unlike typical kinases, TRIB1 lacks catalytic activity due to evolutionary loss of critical ATP-binding residues but retains structural motifs enabling protein-protein interactions. It functions as a signaling scaffold/modulator, regulating key pathways such as MAPK/ERK, PI3K/AKT, and NF-κB, with roles in cell proliferation, differentiation, apoptosis, and metabolic homeostasis.
TRIB1 is implicated in diverse physiological and pathological processes. Studies link its dysregulation to cancers (e.g., leukemia, liver cancer), metabolic disorders (e.g., dyslipidemia), and inflammatory diseases (atherosclerosis). Notably, it interacts with E3 ubiquitin ligases like COP1 to mediate degradation of transcription factors (C/EBPα, p65), influencing myeloid differentiation and inflammatory responses. Its involvement in lipid metabolism regulation, particularly through APOA5 modulation, highlights therapeutic potential for cardiovascular diseases.
Recombinant human TRIB1 protein is typically produced using *E. coli* or mammalian expression systems for functional studies. Structural analyses reveal an N-terminal pseudokinase domain and C-terminal MEK1-binding domain, crucial for its regulatory functions. As a research tool, recombinant TRIB1 aids in elucidating molecular mechanisms of disease and identifying therapeutic targets. Despite progress, its context-dependent roles and pleiotropic signaling effects warrant further investigation to harness its clinical potential.
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