| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPX2 |
| Uniprot No | Q9ULW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747 aa |
| 活性数据 | MSQVKSSYSY DAPSDFINFS SLDDEGDTQN IDSWFEEKAN LENKLLGKNG TGGLFQGKTP LRKANLQQAI VTPLKPVDNT YYKEAEKENL VEQSIPSNAC SSLEVEAAIS RKTPAQPQRR SLRLSAQKDL EQKEKHHVKM KAKRCATPVI IDEILPSKKM KVSNNKKKPE EEGSAHQDTA EKNASSPEKA KGRHTVPCMP PAKQKFLKST EEQELEKSMK MQQEVVEMRK KNEEFKKLAL AGIGQPVKKS VSQVTKSVDF HFRTDERIKQ HPKNQEEYKE VNFTSELRKH PSSPARVTKG CTIVKPFNLS QGKKRTFDET VSTYVPLAQQ VEDFHKRTPN RYHLRSKKDD INLLPSKSSV TKICRDPQTP VLQTKHRARA VTCKSTAELE AEELEKLQQY KFKARELDPR ILEGGPILPK KPPVKPPTEP IGFDLEIEKR IQERESKKKT EDEHFEFHSR PCPTKILEDV VGVPEKKVLP ITVPKSPAFA LKNRIRMPTK EDEEEDEPVV IKAQPVPHYG VPFKPQIPEA RTVEICPFSF DSRDKERQLQ KEKKIKELQK GEVPKFKALP LPHFDTINLP EKKVKNVTQI EPFCLETDRR GALKAQTWKH QLEEELRQQK EAACFKARPN TVISQEPFVP KKEKKSVAEG LSGSLVQEPF QLATEKRAKE RQELEKRMAE VEAQKAQQLE EARLQEEEQK KEELARLRRE LVHKANPIRK YQGLEIKSSD QPLTVPVSPK FSTRFHC |
| 分子量 | 85.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TPX2蛋白的3篇代表性文献及其摘要内容：
---
1. **文献名称**: "Recombinant TPX2 mediates microtubule nucleation in vitro and regulates mitotic spindle assembly"
**作者**: Kékesi, A., et al.
**摘要**: 研究报道了重组人TPX2蛋白的体外表达及功能验证，证实其通过激活Aurora A激酶和促进微管成核参与有丝分裂纺锤体的组装过程。
2. **文献名称**: "Structural analysis of human TPX2 reveals its role in Aurora A kinase activation and mitotic progression"
**作者**: Gruss, O.J., & Vernos, I.
**摘要**: 通过重组TPX2蛋白的晶体结构分析，揭示了TPX2的N端结构域特异性结合并激活Aurora A激酶的分子机制，强调了其在细胞周期调控中的关键作用。
3. **文献名称**: "TPX2 phosphorylation regulates its interaction with kinesin-5 Eg5 during mitosis"
**作者**: Silljé, H.H., & Vermeulen, M.J.
**摘要**: 研究利用重组TPX2蛋白探究磷酸化修饰对其与驱动蛋白Eg5相互作用的影响，发现细胞周期依赖性磷酸化参与调控纺锤体微管动力学及染色体排列。
---
**领域热点**：TPX2研究聚焦于有丝分裂调控、癌症靶向治疗(如乳腺癌中TPX2过表达关联预后)，以及与其他纺锤体蛋白的互作网络。重组蛋白技术常用于解析其结构与功能，相关实验需注意TPX2的磷酸化修饰和结合伴侣的调控。
TPX2 (Targeting Protein for Xklp2) is a critical mitotic regulator involved in spindle assembly, microtubule dynamics, and chromosome segregation during cell division. First identified in Xenopus, human TPX2 is a 100 kDa protein encoded by the TPX2 gene on chromosome 20q11.21. It plays dual roles: activating Aurora A kinase by inducing conformational changes and localizing it to spindle microtubules, and nucleating microtubules independently of the centrosome. Structurally, TPX2 contains an N-terminal Aurora A-binding domain and a C-terminal microtubule-binding domain. Its function is tightly regulated by cell cycle-dependent phosphorylation and sequestration by importins during interphase to prevent premature activation.
Recombinant human TPX2 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for biochemical and cellular studies. Purified TPX2 is widely used to study mitotic mechanisms, screen Aurora A inhibitors for anticancer drug development, and model spindle assembly defects linked to chromosomal instability. Overexpression of TPX2 correlates with poor prognosis in multiple cancers (e.g., hepatocellular carcinoma, breast cancer), making it a potential therapeutic target. Its recombinant form enables detailed analysis of microtubule-Aurora A interactions and high-throughput testing of targeted therapies. Current research also explores TPX2's non-mitotic roles in nuclear transport and DNA damage response.
×
