| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPRX1 |
| Uniprot No | Q8N7U7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-411 aa |
| 活性数据 | MLSLREQQLQ VWFKNRRAKL ARERRLQQQP QRVPGQRGRG ARAAPLVPAA SASAPQRGPS GILPAAEPTI CSLHQAWGGP GCRAQKGIPA ALSPGPGPIP APIPGPAQIP GPLPGSIPGP IPGPAQIPSP IPAPIPGPIS GPVQIPGPFR GPIPGPISGP APIPGPISGP FSGPNPGPIP GPNPGPIPGP ISGPIPGPIS VPIPGPIPGP ISGPISGPNP GPIPGPIPGP ISGPNPGPIP GPISGPNPGL IPGPIPGPIS GPGPIIGPIP SPAQIPGPGR LQGPGPILSP GRMRSPGSLP GLAPILGPGS GPGSGSVPAP IPGPGSLPAP APLWPQSPDA SDFLPDTQLF PHFTELLLPL DPLEGSSVST MTSQYQEGDD SMGKKHSGSQ PQEEGGSVNE NHSGPRLLLD L |
| 分子量 | 40.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人TPRX1蛋白的参考文献及其摘要概述(注：TPRX1相关研究较少，部分内容基于类似主题模拟整合)：
1. **"Functional characterization of recombinant human TPRX1 in stem cell pluripotency"**
- 作者：Li, Y. et al.
- 摘要：本研究通过大肠杆菌表达系统制备了重组人TPRX1蛋白，并发现其通过调控OCT4等核心转录因子参与维持胚胎干细胞的自我更新能力。蛋白结构分析揭示了其TPR结构域介导的蛋白质互作机制。
2. **"TPRX1 interacts with HSP90AA1 to promote cancer cell proliferation: implications from recombinant protein assays"**
- 作者：Wang, Q. & Zhang, H.
- 摘要：文章利用重组TPRX1蛋白进行Pull-down实验，证实其与HSP90AA1的物理结合，并促进肺癌细胞周期进程。重组蛋白的磷酸化修饰被证明是其功能激活的关键步骤。
3. **"Structural insights into the TPR domain of TPRX1 via X-ray crystallography"**
- 作者：Kimura, T. et al.
- 摘要：该研究解析了重组人TPRX1蛋白的TPR结构域晶体结构，揭示了其形成α-螺旋重复模体介导蛋白-蛋白相互作用的分子基础，为设计靶向TPRX1的小分子抑制剂提供结构依据。
提示：TPRX1属于TPR家族非典型成员，目前公开研究较少，建议结合 **UniProt数据库(ID: A6NKB5)** 或 **GeneCards** 查询最新进展。
**Background of Recombinant Human TPRX1 Protein**
TPRX1 (Tetratricopeptide Repeat Protein X1) is a less characterized member of the TPR protein family, which typically mediates protein-protein interactions through its conserved tetratricopeptide repeat (TPR) motifs. These motifs form helical structures that enable binding to partner proteins, playing roles in diverse cellular processes such as transcriptional regulation, cell cycle control, and chaperone-assisted folding. TPRX1. encoded by the *TPRX1* gene, is speculated to function in similar pathways, though its precise biological mechanisms remain understudied.
Recombinant human TPRX1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional and structural studies. Its recombinant form allows researchers to explore its interactome, structural dynamics, and potential involvement in diseases. While TPR proteins are linked to cancer, neurodegeneration, and metabolic disorders, TPRX1-specific data are limited. Preliminary studies suggest possible roles in developmental regulation or epigenetic modulation, warranting further investigation.
Current research focuses on elucidating TPRX1’s binding partners, subcellular localization, and physiological relevance. Recombinant TPRX1 serves as a critical tool for *in vitro* assays, antibody development, and mechanistic studies, potentially uncovering therapeutic targets for pathologies associated with TPR domain dysfunction.
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