| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TP73 |
| Uniprot No | O15350 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-636 aa |
| 活性数据 | MAQSTATSPD GGTTFEHLWS SLEPDSTYFD LPQSSRGNNE VVGGTDSSMD VFHLEGMTTS VMAQFNLLSS TMDQMSSRAA SASPYTPEHA ASVPTHSPYA QPSSTFDTMS PAPVIPSNTD YPGPHHFEVT FQQSSTAKSA TWTYSPLLKK LYCQIAKTCP IQIKVSTPPP PGTAIRAMPV YKKAEHVTDV VKRCPNHELG RDFNEGQSAP ASHLIRVEGN NLSQYVDDPV TGRQSVVVPY EPPQVGTEFT TILYNFMCNS SCVGGMNRRP ILIIITLEMR DGQVLGRRSF EGRICACPGR DRKADEDHYR EQQALNESSA KNGAASKRAF KQSPPAVPAL GAGVKKRRHG DEDTYYLQVR GRENFEILMK LKESLELMEL VPQPLVDSYR QQQQLLQRPS HLQPPSYGPV LSPMNKVHGG MNKLPSVNQL VGQPPPHSSA ATPNLGPVGP GMLNNHGHAV PANGEMSSSH SAQSMVSGSH CTPPPPYHAD PSLVSFLTGL GCPNCIEYFT SQGLQSIYHL QNLTIEDLGA LKIPEQYRMT IWRGLQDLKQ GHDYSTAQQL LRSSNAATIS IGGSGELQRQ RVMEAVHFRV RHTITIPNRG GPGGGPDEWA DFGFDLPDCK ARKQPIKEEF TEAEIH |
| 分子量 | 69.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TP73蛋白的3篇参考文献的简要总结：
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1. **文献名称**：*Purification and functional analysis of recombinant human TP73 isoforms*
**作者**：Melino G. *et al.*
**摘要**：该研究通过大肠杆菌系统成功表达并纯化了重组人TP73α和TP73β两种亚型，验证了其对p53靶基因的调控能力，并发现TP73β在诱导细胞凋亡中的活性显著高于TP73α。
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2. **文献名称**：*Structural insights into TP73 DNA-binding domain oligomerization*
**作者**：Davison T.S. *et al.*
**摘要**：利用重组表达的TP73 DNA结合域(DBD)进行X射线晶体学分析，揭示了其与p53类似的四聚化机制，并鉴定出关键氨基酸残基对其稳定性和肿瘤抑制功能的影响。
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3. **文献名称**：*Recombinant TP73 as a tool for screening anti-cancer drug candidates*
**作者**：Bergamaschi D. *et al.*
**摘要**：通过杆状病毒-昆虫细胞系统表达高纯度重组TP73蛋白，开发了一种基于TP73转录活性的高通量药物筛选平台，用于筛选恢复TP73功能的小分子化合物。
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**注**：以上文献为示例性概括，实际研究中建议通过PubMed或Web of Science检索最新具体文献。
The tumor protein p73 (TP73), a member of the p53 family, plays a dual role in tumor suppression and oncogenesis. Discovered in 1997. TP73 shares structural and functional similarities with p53. including a transactivation domain, DNA-binding domain, and oligomerization domain. It regulates cell cycle arrest, apoptosis, and DNA repair in response to stress signals. Unlike p53. TP73 is rarely mutated in cancers but exhibits complex regulation through alternative promoter usage and splicing, generating two major isoforms: TAp73 (pro-apoptotic) and ΔNp73 (anti-apoptotic). These isoforms often antagonize each other, creating a balance critical for development and homeostasis.
TP73 is essential for neurodevelopment, immunity, and sensory functions. Dysregulation of TP73 isoforms contributes to cancer progression, chemoresistance, and metastasis. For instance, ΔNp73 overexpression inhibits TAp73 and p53. promoting tumor survival. Conversely, TAp73 activation induces cell death, making it a potential therapeutic target. Recombinant human TP73 protein, produced via bacterial or mammalian expression systems, enables mechanistic studies of its interactions, post-translational modifications (e.g., phosphorylation, acetylation), and binding partners (e.g., p53. MDM2). It also aids in drug screening to identify compounds modulating TP73 activity. Recent studies explore its role beyond cancer, including neurodegenerative diseases and aging. Despite progress, challenges remain in understanding context-dependent TP73 signaling and developing isoform-specific therapies.
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