| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TORC2 |
| Uniprot No | Q53ET0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-693 aa |
| 活性数据 | MATSGANGPGSATASASNPRKFSEKIALQKQRQAEETAAFEEVMMDIGSTRLQAQKLRLAYTRSSHYGGSLPNVNQIGSGLAEFQSPLHSPLDSSRSTRHHGLVERVQRDPRRMVSPLRRYTRHIDSSPYSPAYLSPPPESSWRRTMAWGNFPAEKGQLFRLPSALNRTSSDSALHTSVMNPSPQDTYPGPTPPSILPSRRGGILDGEMDPKVPAIEENLLDDKHLLKPWDAKKLSSSSSRPRSCEVPGINIFPSPDQPANVPVLPPAMNTGGSLPDLTNLHFPPPLPTPLDPEETAYPSLSGGNSTSNLTHTMTHLGISRGMGLGPGYDAPGLHSPLSHPSLQSSLSNPNLQASLSSPQPQLQGSHSHPSLPASSLARHVLPTTSLGHPSLSAPALSSSSSSSSTSSPVLGAPSYPASTPGASPHHRRVPLSPLSLLAGPADARRSQQQLPKQFSPTMSPTLSSITQGVPLDTSKLSTDQRLPPYPYSSPSLVLPTQPHTPKSLQQPGLPSQSCSVQSSGGQPPGRQSHYGTPYPPGPSGHGQQSYHRPMSDFNLGNLEQFSMESPSASLVLDPPGFSEGPGFLGGEGPMGGPQDPHTFNHQNLTHCSRHGSGPNIILTGDSSPGFSKEIAAALAGVPGFEVSAAGLELGLGLEDELRMEPLGLEGLNMLSDPCALLPDPAVEESFRSDRLQ |
| 分子量 | 99.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TORC2蛋白的示例参考文献，格式为文献名称、作者及摘要概括。如需真实文献，建议通过PubMed或Web of Science查询最新研究：
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1. **"Cryo-EM structure of human mTORC2 reveals its assembly and regulatory mechanisms"**
*作者：Chen et al.*
*摘要*：通过冷冻电镜解析人源mTORC2复合体的高分辨率结构，揭示了其与RICTOR和mTOR等亚基的相互作用机制，为靶向TORC2的药物设计提供结构基础。
2. **"Recombinant human TORC2 expression system enables functional study of Akt phosphorylation"**
*作者：Smith et al.*
*摘要*：开发基于昆虫细胞的重组人TORC2蛋白表达系统，验证其在体外激活Akt Ser473磷酸化的功能，为研究mTORC2信号通路提供工具。
3. **"TORC2 regulates lipid metabolism through recombinant protein-based assays"**
*作者：González et al.*
*摘要*：利用重组人TORC2蛋白，阐明其通过PKC-α调控脂质合成的分子机制，揭示其在代谢性疾病中的潜在作用。
4. **"Yeast-derived recombinant human TORC2 demonstrates conserved regulatory roles in cell survival"**
*作者：Tanaka et al.*
*摘要*：在酵母中高效表达功能性重组人TORC2蛋白，证明其在不同物种间调控细胞存活和应激反应的保守性。
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**说明**：以上为基于TORC2研究方向构造的示例，实际文献需查阅学术数据库。建议结合关键词“recombinant human TORC2”、“mTORC2 structure”或“TORC2 signaling”进一步检索。
Transducer of Regulated CREB (cAMP response element-binding protein) activity 2 (TORC2), also known as CRTC2. is a coactivator protein critical for modulating CREB-mediated transcriptional regulation. It belongs to the TORC family (TORC1. TORC2. TORC3) and plays a pivotal role in energy homeostasis, gluconeogenesis, and stress response pathways. TORC2 is primarily expressed in the liver, pancreas, and adipose tissues. Structurally, it contains conserved N-terminal and C-terminal domains that facilitate interactions with CREB and other transcription factors.
Under low-energy conditions, TORC2 is dephosphorylated and translocates to the nucleus, where it binds CREB to activate target genes like *PGC-1α* and *G6Pase*, driving glucose production. Its activity is tightly regulated by phosphorylation through AMP-activated protein kinase (AMPK) and salt-inducible kinases (SIKs), linking cellular energy status to metabolic adaptations. Dysregulation of TORC2 is implicated in metabolic disorders, including type 2 diabetes, due to aberrant hepatic glucose output, and in certain cancers via CREB-dependent oncogenic signaling.
Recombinant human TORC2 protein is engineered for research applications, enabling studies on CREB-mediated transcription, metabolic pathways, and drug discovery. Produced using bacterial or mammalian expression systems, it retains functional domains for in vitro binding assays or structural analyses. Its use has advanced understanding of glucose metabolism regulation and potential therapeutic targeting in metabolic diseases.
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