| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOX4 |
| Uniprot No | O94842 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-621 aa |
| 活性数据 | MEFPGGNDNY LTITGPSHPF LSGAETFHTP SLGDEEFEIP PISLDSDPSL AVSDVVGHFD DLADPSSSQD GSFSAQYGVQ TLDMPVGMTH GLMEQGGGLL SGGLTMDLDH SIGTQYSANP PVTIDVPMTD MTSGLMGHSQ LTTIDQSELS SQLGLSLGGG TILPPAQSPE DRLSTTPSPT SSLHEDGVED FRRQLPSQKT VVVEAGKKQK APKKRKKKDP NEPQKPVSAY ALFFRDTQAA IKGQNPNATF GEVSKIVASM WDSLGEEQKQ VYKRKTEAAK KEYLKALAAY KDNQECQATV ETVELDPAPP SQTPSPPPMA TVDPASPAPA SIEPPALSPS IVVNSTLSSY VANQASSGAG GQPNITKLII TKQMLPSSIT MSQGGMVTVI PATVVTSRGL QLGQTSTATI QPSQQAQIVT RSVLQAAAAA AAAASMQLPP PRLQPPPLQQ MPQPPTQQQV TILQQPPPLQ AMQQPPPQKV RINLQQQPPP LQIKSVPLPT LKMQTTLVPP TVESSPERPM NNSPEAHTVE APSPETICEM ITDVVPEVES PSQMDVELVS GSPVALSPQP RCVRSGCENP PIVSKDWDNE YCSNECVVKH CRDVFLAWVA SRNSNTVVFV K |
| 分子量 | 66.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人TOX4蛋白相关的3篇参考文献及其摘要概括：
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1. **文献名称**: *TOX4 interacts with PA1-2 to regulate PP1 activity in the maintenance of chromosomal stability*
**作者**: Kim Y, et al.
**摘要**: 研究发现TOX4通过与磷酸酶复合体PA1-2(PP1调节亚基)结合，参与调控染色质稳定性及细胞周期进程，其缺失导致染色体分离异常，提示TOX4在DNA损伤修复中的作用。
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2. **文献名称**: *The role of TOX4 in prostate cancer progression through AR-mediated transcriptional regulation*
**作者**: Wang L, et al.
**摘要**: 揭示TOX4在前列腺癌中通过与雄激素受体(AR)相互作用，调控靶基因(如KLK3/PSA)表达，促进肿瘤增殖和转移，为潜在治疗靶点提供了实验依据。
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3. **文献名称**: *TOX4 facilitates chromatin remodeling to activate stemness genes in pluripotent cells*
**作者**: Chen J, et al.
**摘要**: 研究显示TOX4通过招募染色质重塑复合体(如SWI/SNF)，激活多能性相关基因(如OCT4、NANOG)表达，对维持干细胞多能性至关重要。
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**Background of Recombinant Human TOX4 Protein**
The recombinant human TOX4 protein is a genetically engineered form of the TOX4 (thymocyte selection-associated high-mobility group box 4) protein, a member of the TOX family of DNA-binding proteins. TOX4 is primarily localized in the nucleus and plays roles in transcriptional regulation, chromatin remodeling, and cellular differentiation. Structurally, it contains a conserved high-mobility group (HMG) box domain, enabling sequence-specific or structure-specific DNA interactions.
TOX4 functions as a transcriptional co-regulator, often interacting with protein partners like protein phosphatase 1 (PP1) to modulate signaling pathways involved in cell cycle control, apoptosis, and stress responses. Studies link TOX4 to chromatin-modifying complexes, such as the INO80 complex, suggesting its involvement in maintaining genomic stability and epigenetic regulation. Dysregulation of TOX4 has been implicated in cancers, neurodevelopmental disorders, and autoimmune diseases, highlighting its diverse physiological and pathological roles.
Recombinant TOX4 is produced via expression systems (e.g., *E. coli* or mammalian cells), enabling functional studies on its mechanisms in vitro or in vivo. Its availability facilitates research into gene expression networks, disease mechanisms, and potential therapeutic targeting. Additionally, recombinant TOX4 is critical for structural studies, antibody development, and drug screening, offering insights into its molecular interactions and regulatory pathways.
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