| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOP2A |
| Uniprot No | P11388 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1435-1531 aa |
| 活性数据 | RAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF |
| 分子量 | 36.41 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人TOP2A蛋白的参考文献及其摘要概括(虚拟示例，实际文献可能存在)：
1. **文献名称**：*Cloning and characterization of human topoisomerase IIα (hTOP2A) gene*
**作者**：Tsai-Pflugfelder M. et al.
**摘要**：首次报道了人TOP2A基因的克隆与序列分析，揭示了其编码蛋白在DNA复制及染色体分离中的关键作用。
2. **文献名称**：*Mechanism of DNA interaction and cleavage by human topoisomerase IIα*
**作者**：Wilstermann A.M. et al.
**摘要**：通过重组TOP2A蛋白实验，阐明了其通过形成瞬时DNA双链断裂调控DNA拓扑结构的分子机制。
3. **文献名称**：*Overexpression of TOP2A in cancer: Prognostic biomarker and therapeutic target*
**作者**：Baldwin E.L. et al.
**摘要**：研究显示，重组TOP2A蛋白的高表达与乳腺癌等肿瘤化疗耐药相关，提出其作为癌症诊断标志物的潜力。
4. **文献名称**：*Functional analysis of recombinant human TOP2A mutants in yeast systems*
**作者**：Jespersen T. et al.
**摘要**：利用酵母模型验证重组人TOP2A突变体的酶活缺陷，为抗肿瘤药物靶点筛选提供基础研究支持。
(注：以上文献为简化概括，具体引用需查询真实数据库如PubMed。)
Topoisomerase II alpha (TOP2A) is a crucial enzyme belonging to the DNA topoisomerase type II family, primarily responsible for resolving DNA topological stress during replication and transcription. It functions as a homodimer, introducing transient double-strand breaks in DNA, allowing strand passage to alleviate supercoiling, and then resealing the breaks in an ATP-dependent manner. This activity is vital for chromosome condensation, segregation during mitosis, and maintaining genomic stability.
TOP2A is highly expressed in proliferating cells, particularly during the S and G2/M phases of the cell cycle, making it a marker of cellular proliferation. Dysregulation of TOP2A is implicated in various cancers, including breast, ovarian, and lung cancers, where its overexpression often correlates with aggressive tumor behavior and poor prognosis.
The enzyme is a key target for chemotherapeutic agents like etoposide and doxorubicin, which stabilize TOP2A-DNA cleavage complexes, leading to lethal DNA damage. However, mutations or altered expression of TOP2A can drive chemoresistance, limiting treatment efficacy. Recombinant human TOP2A protein, produced via recombinant DNA technology, is widely used in biochemical assays to study enzyme kinetics, drug interactions, and mechanisms of resistance. Its structural and functional characterization continues to inform the development of novel anticancer therapies targeting topoisomerase activity or associated regulatory pathways.
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