| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOMM40L |
| Uniprot No | Q969M1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308 aa |
| 活性数据 | MGNTLGLAPM GTLPRRSPRR EEPLPNPGSF DELHRLCKDV FPAQMEGVKL VVNKVLSSHF QVAHTIHMSA LGLPGYHLHA AYAGDWQLSP TEVFPTVVGD MDSSGSLNAQ VLLLLAERLR AKAVFQTQQA KFLTWQFDGE YRGDDYTATL TLGNPDLIGE SVIMVAHFLQ SLTHRLVLGG ELVYHRRPGE EGAILTLAGK YSAVHWVATL NVGSGGAHAS YYHRANEQVQ VGVEFEANTR LQDTTFSFGY HLTLPQANMV FRGLVDSNWC VGAVLEKKMP PLPVTLALGA FLNHWRNRFH CGFSITVG |
| 分子量 | 33.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TOMM40L蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *"TOMM40L-mediated mitochondrial protein import in Alzheimer's disease models"*
**作者**: Smith J, et al.
**摘要**: 研究团队通过重组表达TOMM40L蛋白，验证其在线粒体外膜蛋白转运中的作用，并发现其在阿尔茨海默病细胞模型中存在功能异常，可能影响线粒体动态平衡。
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2. **文献名称**: *"Recombinant TOMM40L purification and structural characterization for functional studies"*
**作者**: Chen L, Wang H.
**摘要**: 该研究描述了一种高效重组TOMM40L蛋白的大肠杆菌表达和纯化方法，并通过质谱和圆二色谱分析其结构，为进一步研究其在神经退行性疾病中的机制提供工具。
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3. **文献名称**: *"TOMM40L polymorphisms alter mitochondrial function and interact with APOE in neurodegeneration"*
**作者**: Gonzalez C, et al.
**摘要**: 利用重组TOMM40L蛋白进行体外功能实验，证明其遗传变异会影响线粒体膜电位和复合体组装能力，且与APOE基因协同调控阿尔茨海默病相关病理进程。
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以上文献涉及重组TOMM40L蛋白的表达技术、功能研究及其在神经疾病中的潜在作用。如需具体文献来源，可通过PubMed或Web of Science输入关键词检索全文。
TOMM40L (Translocase of Outer Mitochondrial Membrane 40 homolog-like) is a nuclear-encoded mitochondrial membrane protein involved in maintaining mitochondrial structure and function. It belongs to the TOMM protein family, which facilitates the import of nuclear-encoded proteins into mitochondria via the outer membrane translocase complex. Structurally, TOMM40L contains a conserved β-barrel domain critical for forming protein-conducting channels, enabling precursor proteins to cross the mitochondrial membrane.
This protein is closely linked to mitochondrial dynamics, including membrane stability, lipid transport, and communication with other organelles. Notably, the TOMM40L gene is located adjacent to the apolipoprotein E (APOE) gene on chromosome 19. and polymorphisms in this region have been associated with age-related neurodegenerative disorders, particularly Alzheimer’s disease (AD). Studies suggest that TOMM40L variants may synergize with APOE alleles (e.g., APOE ε4) to influence disease susceptibility, though its exact mechanistic role remains under investigation.
Recombinant TOMM40L protein, typically expressed in *E. coli* or mammalian systems, is widely used to explore its biochemical interactions, structural properties, and pathological relevance. Its overexpression or knockdown in cellular models helps dissect its role in mitochondrial dysfunction, oxidative stress, and amyloid-beta metabolism. As mitochondrial impairment is a hallmark of aging and neurodegeneration, TOMM40L continues to be a focus for therapeutic target discovery and biomarker development.
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