| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRPF18 |
| Uniprot No | Q99633 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342 aa |
| 活性数据 | MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL |
| 分子量 | 39.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRPF18蛋白的3篇示例参考文献(注意部分内容可能基于真实研究背景简化或整合，文献信息为示例性)：
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1. **文献名称**: **"Recombinant Human PRPF18 Facilitates Spliceosome Activation in Vitro"**
**作者**: Müller C, et al.
**摘要**: 本研究在大肠杆菌系统中表达了重组人PRPF18蛋白，证实其通过促进U2 snRNP与pre-mRNA的结合，在剪接体激活阶段起关键作用。体外剪接实验显示PRPF18缺失导致剪接效率显著降低。
2. **文献名称**: **"Structural and Functional Analysis of PRPF18 in Spliceosome Remodeling"**
**作者**: Liu Y, et al.
**摘要**: 利用重组PRPF18蛋白进行冷冻电镜结构解析，揭示了其C端结构域与剪接体组分Prp8的相互作用，提出PRPF18在剪接体解离和再循环中的分子机制。
3. **文献名称**: **"PRPF18 Mutations Disrupt Splicing Fidelity via Impaired Interactions with SF3B1"**
**作者**: Chen X, et al.
**摘要**: 研究通过重组野生型/突变型PRPF18蛋白，结合酵母双杂交和共沉淀实验，发现PRPF18致病突变削弱其与SF3B1的互作，导致异常剪接事件，可能与视网膜变性相关。
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**说明**：以上文献示例综合了PRPF18在剪接中的功能、结构分析及疾病关联研究。实际文献需通过学术数据库(如PubMed、Web of Science)以关键词“PRPF18 recombinant”“pre-mRNA splicing”等进一步验证。若需要具体文献，请提供更详细信息以便精准检索。
PRPF18 (Pre-mRNA Processing Factor 18) is a conserved eukaryotic protein integral to the spliceosome, a dynamic ribonucleoprotein complex responsible for removing introns from precursor messenger RNA (pre-mRNA) during splicing. As a component of the "Bact" spliceosome complex, PRPF18 plays a critical role in facilitating the later stages of splicing, particularly in catalytic activation and intron release. It interacts with other splicing factors, such as PRPF8 and SNRNP200. to stabilize RNA structures and ensure splice site recognition fidelity. Structurally, PRPF18 contains a degenerate G-patch domain, a motif associated with RNA binding and protein-protein interactions, which is essential for its function in spliceosomal remodeling.
Recombinant human PRPF18 protein is produced via heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) for biochemical and functional studies. Its recombinant form enables detailed exploration of spliceosome assembly dynamics, RNA processing mechanisms, and pathological implications. Dysregulation of PRPF18 has been linked to human diseases, including retinitis pigmentosa and myelodysplastic syndromes, highlighting its importance in cellular homeostasis. Current research focuses on elucidating its precise molecular interactions and potential therapeutic targeting in splicing-related disorders. As a tool, recombinant PRPF18 aids in reconstructing splicing events in vitro and screening modulators of spliceosomal activity.
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