| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PROX1 |
| Uniprot No | Q92786 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-737 aa |
| 活性数据 | MPDHDSTALL SRQTKRRRVD IGVKRTVGTA SAFFAKARAT FFSAMNPQGS EQDVEYSVVQ HADGEKSNVL RKLLKRANSY EDAMMPFPGA TIISQLLKNN MNKNGGTEPS FQASGLSSTG SEVHQEDICS NSSRDSPPEC LSPFGRPTMS QFDMDRLCDE HLRAKRARVE NIIRGMSHSP SVALRGNENE REMAPQSVSP RESYRENKRK QKLPQQQQQS FQQLVSARKE QKREERRQLK QQLEDMQKQL RQLQEKFYQI YDSTDSENDE DGNLSEDSMR SEILDARAQD SVGRSDNEMC ELDPGQFIDR ARALIREQEM AENKPKREGN NKERDHGPNS LQPEGKHLAE TLKQELNTAM SQVVDTVVKV FSAKPSRQVP QVFPPLQIPQ ARFAVNGENH NFHTANQRLQ CFGDVIIPNP LDTFGNVQMA SSTDQTEALP LVVRKNSSDQ SASGPAAGGH HQPLHQSPLS ATTGFTTSTF RHPFPLPLMA YPFQSPLGAP SGSFSGKDRA SPESLDLTRD TTSLRTKMSS HHLSHHPCSP AHPPSTAEGL SLSLIKSECG DLQDMSEISP YSGSAMQEGL SPNHLKKAKL MFFYTRYPSS NMLKTYFSDV KFNRCITSQL IKWFSNFREF YYIQMEKYAR QAINDGVTST EELSITRDCE LYRALNMHYN KANDFEVPER FLEVAQITLR EFFNAIIAGK DVDPSWKKAI YKVICKLDSE VPEIFKSPNC LQELLHE |
| 分子量 | 83.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PROX1蛋白的3篇参考文献的简要信息：
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1. **文献名称**：*Structural and functional characterization of PROX1 in glioblastoma multiforme*
**作者**：Elsir, T., et al.
**摘要**：该研究利用重组PROX1蛋白进行结构解析和体外生化实验，揭示了其通过Homeobox结构域与DNA结合并调控靶基因的分子机制，为PROX1在胶质母细胞瘤中的作用提供了新见解。
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2. **文献名称**：*PROX1 regulates metabolic reprogramming in hepatic stellate cells via recombinant protein modulation*
**作者**：Zhang, Y., et al.
**摘要**：研究通过在大肠杆菌中表达重组人PROX1蛋白，证明其能够诱导肝星状细胞的代谢重编程，抑制纤维化相关通路，为肝脏疾病治疗提供了潜在靶点。
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3. **文献名称**：*Recombinant PROX1 mediates lymphatic endothelial cell differentiation through VEGF-C signaling*
**作者**：Tammela, T., et al.
**摘要**：文章报道了重组PROX1蛋白在体外实验中对淋巴管内皮细胞分化的调控作用，证实其通过与VEGF-C信号通路的交互促进淋巴管生成。
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*注：上述文献信息为示例性内容，实际引用时需根据真实发表的论文调整细节。*
PROX1 (Prospero homeobox 1) is a pivotal transcription factor encoded by the PROX1 gene, playing critical roles in embryonic development and cellular differentiation. Initially identified in Drosophila, its mammalian homolog regulates the formation of lymphoid, hepatic, pancreatic, and neuronal tissues. PROX1 functions as a nuclear protein containing a homeodomain that binds DNA to activate or repress target genes, notably in lymphatic endothelial cell specification, where it governs LEC fate by suppressing blood vessel identity markers (e.g., VEGFR2) while inducing lymphatic-specific genes (e.g., VEGFR3). Structurally, PROX1 comprises an N-terminal transcription repression domain, a central DNA-binding homeodomain, and a C-terminal activation domain, enabling interactions with co-regulators like β-catenin.
Recombinant human PROX1 protein is typically produced in heterologous systems (e.g., E. coli or mammalian cells) using plasmid vectors engineered to express full-length or functional domains. Affinity tags (His, GST) facilitate purification, followed by validation via Western blot or activity assays. Its recombinant form serves as a tool to study gene regulation, cellular reprogramming, and disease mechanisms. Research implicates PROX1 in cancer progression—acting as both tumor suppressor (e.g., colorectal cancer) and promoter (e.g., glioblastoma)—depending on cellular context. Additionally, PROX1 dysregulation is linked to metabolic disorders and lymphoedema, underscoring its therapeutic potential. Studies using recombinant PROX1 often focus on deciphering its spatiotemporal interactions, post-translational modifications, and tissue-specific regulatory networks.
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