| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE4C |
| Uniprot No | Q08493 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-712 aa |
| 活性数据 | MENLGVGEGAEACSRLSRSRGRHSMTRAPKHLWRQPRRPIRIQQRFYSDPDKSAGCRERDLSPRPELRKSRLSWPVSSCRRFDLENGLSCGRRALDPQSSPGLGRIMQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAALARQQCLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILNRELTHLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGVQTDQEEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYHANVAYHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYNDASVLENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVLLLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTASVEKSQVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTLEEAEEEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT |
| 分子量 | 106.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and purification of recombinant human PDE4C in Escherichia coli for structural studies"**
- Author: Zhang, L. et al.
- 摘要：本研究通过在大肠杆菌中优化表达条件，成功纯化了重组人PDE4C蛋白，并利用X射线晶体学解析了其三维结构，揭示了其催化结构域的关键氨基酸残基。
2. **"Functional characterization of PDE4C isoforms and their regulation by cyclic AMP signaling"**
- Author: Bolger, G.B. et al.
- 摘要：通过分析重组表达的PDE4C不同剪接变体，发现不同异构体在亚细胞定位和cAMP水解活性上的差异，提示其在细胞信号通路中的特异性调控作用。
3. **"Development of PDE4C-specific inhibitors with anti-inflammatory activity using high-throughput screening"**
- Author: Wang, Y. et al.
- 摘要：基于重组人PDE4C蛋白的酶活性检测平台，筛选出新型选择性抑制剂，体外实验验证其对单核细胞炎症因子释放的抑制作用，具有潜在治疗慢性阻塞性肺病(COPD)的应用价值。
4. **"Role of PDE4C in neuronal cAMP metabolism and synaptic plasticity"**
- Author: Richter, W. et al.
- 摘要：利用重组PDE4C蛋白及基因敲除模型，揭示其在海马神经元中调控cAMP水平的作用，影响长期记忆形成的分子机制。
(注：上述文献为示例，实际引用需根据真实研究调整。)
**Background of Recombinant Human PDE4C Protein**
Recombinant human PDE4C (phosphodiesterase 4C) is a engineered version of the PDE4C enzyme, belonging to the phosphodiesterase 4 (PDE4) family that hydrolyzes cyclic adenosine monophosphate (cAMP), a key secondary messenger in cellular signaling. PDE4C, encoded by the *PDE4C* gene, is one of four subtypes (PDE4A-D) sharing a conserved catalytic domain but differing in regulatory regions and tissue distribution. It plays roles in modulating cAMP-mediated pathways involved in inflammation, neural function, and cell proliferation.
Structurally, PDE4C contains two N-terminal upstream conserved regions (UCR1 and UCR2) that regulate enzyme activity and interactions, alongside a catalytic domain responsible for cAMP degradation. Its expression is detected in the brain, heart, and skeletal muscle, suggesting tissue-specific regulatory functions. Dysregulation of PDE4C has been implicated in neurological disorders, cancers, and inflammatory diseases, making it a potential therapeutic target.
Recombinant PDE4C is typically produced using bacterial or mammalian expression systems for biochemical and pharmacological studies. Its recombinant form enables structural analysis, inhibitor screening, and mechanism exploration, aiding in the development of PDE4C-selective drugs. Unlike pan-PDE4 inhibitors (e.g., rolipram), which cause side effects like nausea, targeting PDE4C subtype-specific regions may enhance therapeutic precision. Current research focuses on elucidating its isoform-specific roles and optimizing inhibitors for neurodegenerative and immune-related conditions.
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