| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCD7 |
| Uniprot No | Q8N8D1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485 aa |
| 活性数据 | MALPPFFGQG RPGPPPPQPP PPAPFGCPPP PLPSPAFPPP LPQRPGPFPG ASAPFLQPPL ALQPRASAEA SRGGGGAGAF YPVPPPPLPP PPPQCRPFPG TDAGERPRPP PPGPGPPWSP RWPEAPPPPA DVLGDAALQR LRDRQWLEAV FGTPRRAGCP VPQRTHAGPS LGEVRARLLR ALRLVRRLRG LSQALREAEA DGAAWVLLYS QTAPLRAELA ERLQPLTQAA YVGEARRRLE RVRRRRLRLR ERAREREAER EAEAARAVER EQEIDRWRVK CVQEVEEKKR EQELKAAADG VLSEVRKKQA DTKRMVDILR ALEKLRKLRK EAAARKGVCP PASADETFTH HLQRLRKLIK KRSELYEAEE RALRVMLEGE QEEERKRELE KKQRKEKEKI LLQKREIESK LFGDPDEFPL AHLLEPFRQY YLQAEHSLPA LIQIRHDWDQ YLVPSDHPKG NFVPQGWVLP PLPSNDIWAT AVKLH |
| 分子量 | 54.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设构造的3篇与重组人PDCD7(可能对应AIFM2)蛋白相关的参考文献示例，供参考：
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**1. 文献名称**：*Recombinant human PDCD7/AIFM2 promotes mitochondrial apoptosis in cancer cells*
**作者**：Zhang Y. et al.
**摘要**：本研究成功在大肠杆菌中表达了可溶性的重组人PDCD7蛋白，并证实其通过线粒体途径诱导HeLa细胞凋亡。Western blot显示PDCD7激活Caspase-9依赖性凋亡通路，为靶向癌症治疗提供潜在策略。
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**2. 文献名称**：*Structural insights into the redox-dependent function of PDCD7/AIFM2*
**作者**：Wang L. et al.
**摘要**：通过X射线晶体学解析了重组PDCD7蛋白的三维结构(2.8 Å)，揭示其依赖NADH的氧化还原活性位点。突变实验表明，该位点对其促凋亡功能至关重要，提示PDCD7在细胞氧化应激中扮演双重角色。
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**3. 文献名称**：*PDCD7/AIFM2 knockdown attenuates neuronal death in a Parkinson’s disease model*
**作者**：Chen X. et al.
**摘要**：采用杆状病毒系统表达重组PDCD7蛋白，并利用siRNA敲低实验验证其在帕金森模型中的神经保护作用。结果表明PDCD7通过调控线粒体膜电位影响多巴胺能神经元存活。
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**备注**：以上文献为假设性示例，实际研究中PDCD7可能与AIFM2(Apoptosis-Inducing Factor Mitochondrial 2)为同一蛋白，建议通过权威数据库(如UniProt)或PubMed进一步核实最新进展。
**Background of Recombinant Human PDCD7 Protein**
Programmed cell death 7 (PDCD7), also known as programmed death-related protein 7. is a conserved eukaryotic protein implicated in apoptosis regulation and cellular stress responses. While its exact molecular mechanisms remain under investigation, PDCD7 is hypothesized to interact with components of the mitochondrial apoptosis pathway, potentially influencing cell cycle progression and DNA damage repair. Studies suggest PDCD7 may function as a tumor suppressor, with reduced expression observed in certain cancers, correlating with poor prognosis.
Recombinant human PDCD7 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce functional, purified protein for research. This enables detailed characterization of its biochemical properties, structural features (e.g., conserved N-terminal domains), and interactions with signaling molecules like caspases or Bcl-2 family proteins. Applications include elucidating PDCD7's role in apoptosis, autophagy, and cancer biology, as well as screening for therapeutic agents targeting apoptosis-related diseases.
Despite progress, PDCD7's full interactome and regulatory pathways remain incompletely defined, necessitating further exploration to clarify its physiological and pathological significance.
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