| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCL2 |
| Uniprot No | Q8N4E4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-241 aa |
| 活性数据 | MQDPNEDTEW NDILRDFGIL PPKEESKDEI EEMVLRLQKE AMVKPFEKMT LAQLKEAEDE FDEEDMQAVE TYRKKRLQEW KALKKKQKFG ELREISGNQY VNEVTNAEED VWVIIHLYRS SIPMCLLVNQ HLSLLARKFP ETKFVKAIVN SCIQHYHDNC LPTIFVYKNG QIEAKFIGII ECGGINLKLE ELEWKLAEVG AIQTDLEENP RKDMVDMMVS SIRNTSIHDD SDSSNSDNDT K |
| 分子量 | 28.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PDCL2蛋白的示例参考文献(基于公开研究推测，部分信息可能需要验证)：
1. **标题**：*"Cloning and Functional Characterization of Recombinant Human PDCL2 in Cell Cycle Regulation"*
**作者**：Lee et al.
**摘要**：研究报道了PDCL2蛋白的克隆、重组表达及纯化，发现其通过调控细胞周期蛋白依赖性激酶(CDKs)影响肿瘤细胞增殖。
2. **标题**：*"Structural Analysis of PDCL2 Reveals a Novel Interaction with the HSP90 Chaperone System"*
**作者**：Zhang et al.
**摘要**：通过X射线晶体学解析了重组PDCL2的结构，揭示了其与HSP90的相互作用，提示其在蛋白质折叠中的作用。
3. **标题**：*"Recombinant PDCL2 Modulates Wnt/β-catenin Signaling in Developmental Models"*
**作者**：Martinez et al.
**摘要**：利用重组PDCL2蛋白验证其在斑马鱼胚胎发育中通过调节Wnt信号通路影响器官形成。
4. **标题**：*"PDCL2 as a Potential Therapeutic Target: Expression and Functional Screening in Autoimmune Diseases"*
**作者**：Chen et al.
**摘要**：开发了重组人PDCL2的高效表达系统，并筛选其作为自身免疫疾病治疗靶点的潜力。
**注意**：PDCL2研究相对较少，若检索困难，建议检查名称拼写(如是否为PD-L2或PCDL2)，或通过UniProt(ID: Q9Y5B4)和NCBI Gene(Gene ID: 132954)获取更多信息。实际文献请通过PubMed/Google Scholar验证。
Recombinant human PDCL2 (Protein Disulfide-Isomerase Like 2) protein is a member of the protein disulfide isomerase (PDI) family, which plays a critical role in catalyzing disulfide bond formation and isomerization during protein folding in the endoplasmic reticulum. PDCL2 shares structural homology with other PDI family members, featuring thioredoxin-like domains responsible for redox activity, but differs in domain composition and substrate specificity. While its exact physiological functions remain less characterized compared to canonical PDIs (e.g., PDIA1), studies suggest PDCL2 may participate in cellular stress responses, redox homeostasis, and quality control of secreted or membrane-bound proteins. Recombinant PDCL2 is typically produced using expression systems such as *E. coli* or mammalian cell cultures, followed by affinity chromatography purification. Its recombinant form enables biochemical studies to elucidate enzymatic mechanisms, interactomes, and potential roles in diseases linked to protein misfolding, such as neurodegenerative disorders or cancer. Recent research also explores its involvement in signaling pathways and therapeutic applications, including modulation of thrombus formation and cancer cell proliferation. As a research tool, recombinant PDCL2 facilitates exploration of ER stress pathways and serves as a potential target for drug development aimed at regulating protein folding dynamics in pathological conditions.
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