| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDCD8 |
| Uniprot No | O95831 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 103-612 aa |
| 活性数据 | GLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHE |
| 分子量 | 71.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PDCD8(即AIFM1.凋亡诱导因子1)蛋白的示例参考文献(虚构内容，仅供格式参考)：
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1. **文献名称**: "重组人PDCD8蛋白在大肠杆菌中的高效表达及其促凋亡功能研究"
**作者**: 李明等
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人PDCD8蛋白，通过体外实验证实其能够诱导HeLa细胞凋亡，揭示了其依赖线粒体途径的凋亡调控机制。
2. **文献名称**: "Structural and functional analysis of AIFM1 in neurodegenerative diseases"
**作者**: Smith J, et al.
**摘要**: 解析了重组人AIFM1蛋白的晶体结构，并发现其突变体在帕金森病模型中影响神经元凋亡，提示其在神经退行性疾病中的潜在作用。
3. **文献名称**: "Recombinant PDCD8 enhances chemotherapy sensitivity in breast cancer cells"
**作者**: Zhang Y, et al.
**摘要**: 通过重组人PDCD8蛋白与化疗药物联用，显著提高乳腺癌细胞对药物的敏感性，表明其作为辅助治疗靶点的潜力。
4. **文献名称**: "AIFM1-mediated apoptosis and its role in myocardial ischemia-reperfusion injury"
**作者**: Wang H, et al.
**摘要**: 在小鼠模型中证明，重组AIFM1蛋白通过调节心肌细胞凋亡加重缺血再灌注损伤，为心血管疾病治疗提供新视角。
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*注：以上文献名称与内容为示例，实际引用时需通过学术数据库(如PubMed、Web of Science)核实真实文献。建议使用关键词“recombinant AIFM1”或“PDCD8 apoptosis”进行检索。*
Programmed Cell Death 8 (PDCD8), commonly known as Apoptosis-Inducing Factor (AIF), is a mitochondrial flavoprotein with dual roles in cellular homeostasis and apoptosis. Initially identified as a caspase-independent mediator of apoptosis, AIF translocates from mitochondria to the nucleus under apoptotic stimuli, triggering chromatin condensation and DNA fragmentation. Beyond its pro-death function, AIF also participates in redox regulation through its NADH oxidase activity, supporting mitochondrial respiration and antioxidant defense. Structurally, AIF contains an N-terminal mitochondrial localization signal, a central FAD-binding domain, and a C-terminal oligomerization domain.
Recombinant human PDCD8/AIF protein is produced using genetic engineering techniques (e.g., E. coli or mammalian expression systems) to study its biochemical properties and therapeutic potential. It serves as a critical tool for investigating mitochondrial pathologies, neurodegenerative disorders (e.g., Parkinson’s disease), and cancer mechanisms. Notably, AIF deficiencies or mutations are linked to severe mitochondrial encephalopathies, while its overexpression correlates with chemo-resistance in tumors. Current research explores modulating AIF pathways for neuroprotection or sensitizing cancer cells to treatment. Recombinant AIF variants, including truncated forms lacking the mitochondrial signal peptide, enable precise functional analysis in vitro. Post-translational modifications (e.g., calpain-mediated cleavage) and interactions with heat shock proteins further refine its activity, making recombinant AIF indispensable for deciphering its context-dependent roles in cell survival and death. Studies also employ RNA interference or CRISPR-edited models alongside recombinant AIF to validate its physiological and pathological impacts.
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