| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRLC2 |
| Uniprot No | O14950 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172 aa |
| 活性数据 | MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD |
| 分子量 | 26.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人肌球蛋白调节轻链2(MRLC2/MYL2)蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**: "Phosphorylation and Function of Recombinant Human Myosin Regulatory Light Chain in Smooth Muscle Contraction"
**作者**: Kimura K. et al.
**摘要**: 该研究利用杆状病毒表达系统成功表达了重组人MRLC2蛋白，并证明其在体外能被肌球蛋白轻链激酶(MLCK)磷酸化，揭示了该磷酸化事件在平滑肌收缩中的关键作用。
2. **文献名称**: "Recombinant Expression and Structural Analysis of Human MYL2 for Mechanistic Studies in Cardiomyopathy"
**作者**: Wang Y. et al.
**摘要**: 文章报道了通过大肠杆菌系统高效纯化重组人MRLC2蛋白，结合X射线晶体学解析其结构，为遗传性心肌病相关突变导致的功能异常提供了分子机制解释。
3. **文献名称**: "Regulatory Role of MRLC2 in Cell Migration: Insights from Recombinant Protein Studies"
**作者**: Patel S. & Ito M.
**摘要**: 通过表达重组人MRLC2及其磷酸化突变体，研究证实了MRLC2的磷酸化状态通过调控肌球蛋白II活性，直接影响肿瘤细胞的迁移能力和侵袭过程。
如有需要，可通过PubMed或Web of Science进一步检索DOI及发表年份。建议结合具体研究方向筛选文献。
**Background of Recombinant Human Myosin Regulatory Light Chain 2 (MRLC2) Protein**
Myosin Regulatory Light Chain 2 (MRLC2), also known as MYL2. is a key regulatory component of the myosin II complex, critical for muscle contraction and non-muscle cellular processes such as cytokinesis, cell migration, and maintenance of cell shape. It belongs to the EF-hand calcium-binding protein family and modulates the ATPase activity of myosin II by interacting with its heavy chain. Phosphorylation of MRLC2 at specific residues (e.g., Ser19/Thr18) regulates actomyosin contractility, influencing cellular tension and motility.
Recombinant human MRLC2 protein is produced using expression systems like *E. coli* or mammalian cells, often tagged for purification. Its production enables biochemical studies, structural analysis, and functional assays to dissect the role of myosin II in diseases. Aberrant MRLC2 expression or phosphorylation is linked to cardiovascular disorders (e.g., hypertrophic cardiomyopathy), neurological conditions, and cancer metastasis.
Research applications include investigating cytoskeletal dynamics, drug screening targeting myosin-dependent pathways, and modeling genetic mutations. Recombinant MRLC2 serves as a vital tool for understanding mechanobiology and developing therapies for muscle-related and motility-driven diseases.
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