| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRAP |
| Uniprot No | Q8TCY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172 aa |
| 活性数据 | MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS |
| 分子量 | 45.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRAP蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：Role of MRAP in MC2R Trafficking and Signaling
**作者**：Hinkle, P.M., & Sebag, J.A.
**摘要**：该研究探讨了MRAP蛋白对黑皮质素受体MC2R的调控作用，发现重组人MRAP的表达是MC2R细胞膜定位及ACTH信号通路激活的必要条件，揭示了MRAP在受体功能中的关键辅助角色。
2. **文献名称**：Structural and Functional Analysis of MRAP Dimerization
**作者**：Webber, A.L., et al.
**摘要**：通过重组表达人MRAP蛋白，研究者解析了其独特的反向平行二聚体结构，并证明这种二聚化形式对MC2R的配体结合能力和下游信号传导具有调控作用。
3. **文献名称**：MRAP Interactions with Melanocortin Receptors: Mechanism and Disease Implications
**作者**：Sebag, J.A., et al.
**摘要**：利用重组MRAP蛋白进行体外实验，揭示了其与不同黑皮质素受体(如MC2R和MC4R)的互作特异性，并探讨了MRAP突变导致肾上腺功能不全的分子机制。
注：以上文献为虚拟示例，实际文献需通过PubMed或专业学术数据库检索。建议结合关键词“recombinant human MRAP”或“MRAP protein interaction”进一步查找。
Melanocortin 2 receptor accessory protein (MRAP), first identified in 2005. is a critical regulator of melanocortin receptor signaling, particularly for the melanocortin 2 receptor (MC2R). This single-pass transmembrane protein facilitates MC2R trafficking to the cell surface and enhances its binding to adrenocorticotropic hormone (ACTH), which is essential for adrenal cortisol production. MRAP exists in two splice variants (α and β) and forms antiparallel homodimers through a unique disulfide bond-mediated structure.
Mutations in MRAP are linked to familial glucocorticoid deficiency (FGD), a rare disorder characterized by ACTH resistance and impaired adrenal steroidogenesis. Beyond adrenal function, MRAP’s paralog, MRAP2. has emerged as a key player in energy homeostasis and metabolic regulation, interacting with multiple GPCRs.
Recombinant human MRAP protein, produced via heterologous expression systems like E. coli or mammalian cells, serves as a vital tool for studying receptor interaction mechanisms, modeling adrenal pathologies, and developing therapeutic strategies for endocrine disorders. Its applications extend to exploring novel hormone replacement therapies and targeted interventions for metabolic diseases. Research continues to unravel MRAP's broader roles in cellular trafficking and signaling modulation.
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