| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRGPRD |
| Uniprot No | Q8TDS7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321 aa |
| 活性数据 | MNQTLNSSGTVESALNYSRGSTVHTAYLVLSSLAMFTCLCGMAGNSMVIWLLGFRMHRNPFCIYILNLAAADLLFLFSMASTLSLETQPLVNTTDKVHELMKRLMYFAYTVGLSLLTAISTQRCLSVLFPIWFKCHRPRHLSAWVCGLLWTLCLLMNGLTSSFCSKFLKFNEDRCFRVDMVQAALIMGVLTPVMTLSSLTLFVWVRRSSQQWRRQPTRLFVVVLASVLVFLICSLPLSIYWFVLYWLSLPPEMQVLCFSLSRLSSSVSSSANPVIYFLVGSRRSHRLPTRSLGTVLQQALREEPELEGGETPTVGTNEMGA |
| 分子量 | 42.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRGPRD蛋白研究的示例参考文献(注：以下内容为虚拟示例，仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：**Structural insights into MRGPRD activation by neuropeptides**
**作者**：Chen L. et al. (2022)
**摘要**：通过冷冻电镜解析了MRGPRD与内源性配体β-防御素的复合物结构，揭示了该受体独特的配体结合口袋构象，并阐明了其偏向性信号转导的分子机制。
2. **文献名称**：**MRGPRD mediates nociception in chronic inflammatory pain**
**作者**：Wang Y. et al. (2020)
**摘要**：研究发现，重组MRGPRD在感觉神经元中特异性响应炎症微环境中的促痛肽，通过激活TRPV1通道加剧疼痛信号传导，提示其作为镇痛治疗新靶点的潜力。
3. **文献名称**：**Development of a recombinant MRGPRD-based assay for ligand screening**
**作者**：Kim S. et al. (2021)
**摘要**：构建了重组MRGPRD的高效表达系统，开发了基于β-arrestin招募的体外筛选平台，成功筛选出小分子激动剂和拮抗剂，为药理学研究提供工具。
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如需真实文献，建议在 **PubMed/Google Scholar** 检索关键词：
`MRGPRD recombinant expression`、`MRGPRD signaling`、`MRGPRD pain/itch`。
**Background of Recombinant Human MRGPRD Protein**
MRGPRD (Mas-related G protein-coupled receptor D) is a member of the GPCR family, primarily expressed in sensory neurons, skin cells, and immune cells. It plays roles in itch, pain signaling, and inflammatory responses. Unlike classical GPCRs, MRGPRD interacts with diverse ligands, including antimicrobial peptides, neuropeptides, and small molecules, suggesting involvement in host defense and neuroimmune crosstalk.
Recombinant MRGPRD protein is engineered *in vitro* using biotechnological methods (e.g., mammalian or bacterial expression systems) to study its structure, ligand-binding mechanisms, and downstream signaling pathways. Its production enables exploration of receptor function in physiological and pathological contexts, such as chronic pain, allergic inflammation, and skin disorders.
Research highlights MRGPRD as a potential therapeutic target due to its role in modulating mast cell degranulation and neuronal hypersensitivity. However, its exact physiological roles remain partially unclear, necessitating further studies. Recombinant MRGPRD tools are critical for drug screening and elucidating its interactions with neural and immune systems, offering insights into novel treatments for inflammatory and neuropathic conditions.
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