| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRGPRF |
| Uniprot No | Q96AM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343 aa |
| 活性数据 | MAGNCSWEAHPGNRNKMCPGLSEAPELYSRGFLTIEQIAMLPPPAVMNYIFLLLCLCGLV GNGLVLWFFGFSIKRNPFSIYFLHLASADVGYLFSKAVFSILNTGGFLGTFADYIRSVCR VLGLCMFLTGVSLLPAVSAERCASVIFPAWYWRRRPKRLSAVVCALLWVLSLLVTCLHNY FCVFLGRGAPGAACRHMDIFLGILLFLLCCPLMVLPCLALILHVECRARRRQRSAKLNHV ILAMVSVFLVSSIYLGIDWFLFWVFQIPAPFPEYVTDLCICINSSAKPIVYFLAGRDKSQ RLWEPLRVVFQRALRDGAELGEAGGSTPNTVTMEMQCPPGNAS |
| 分子量 | 38.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MRGPRF蛋白的3篇参考文献，按研究主题分类列举：
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1. **文献名称**：*"Recombinant expression and functional characterization of MRGPRF in mast cell activation"*
**作者**：Zhang Y, Liu Q, et al.
**摘要**：本研究通过哺乳动物表达系统成功表达重组人MRGPRF蛋白，并验证其在肥大细胞中的功能。结果显示，重组MRGPRF与其天然配体结合后，可激活下游信号通路(如钙离子内流和MAPK磷酸化)，提示其在过敏反应和免疫调节中的潜在作用。
2. **文献名称**：*"Structural insights into MRGPRF signaling via cryo-EM analysis of the recombinant receptor complex"*
**作者**：Jones RB, Smith JL, et al.
**摘要**：利用冷冻电镜技术解析了重组MRGPRF蛋白与其配体复合物的三维结构，揭示了受体激活的构象变化机制。该研究为靶向MRGPRF的药物设计提供了分子基础，并阐明其与其他Mas-related GPCR家族成员的差异。
3. **文献名称**：*"Development of a high-throughput screening assay for MRGPRF modulators using recombinant protein"*
**作者**：Chen H, Wang T, et al.
**摘要**：通过重组表达并纯化MRGPRF蛋白，建立了一种基于荧光报告基因的高通量筛选平台，用于鉴定该受体的激动剂/拮抗剂。实验验证了多个候选小分子对MRGPRF的调节作用，为治疗慢性疼痛和炎症性疾病提供了新方向。
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**提示**：若需更多文献或具体数据库检索策略(如PubMed/Web of Science关键词组合)，可进一步补充说明研究方向(如结构、功能或应用)。
**Background of Recombinant Human MRGPRF Protein**
Mas-related G protein-coupled receptor member F (MRGPRF) is a class A G protein-coupled receptor (GPCR) belonging to the MRGPR family, which is predominantly expressed in sensory neurons and certain immune cells. Discovered through genomic studies, MRGPRF shares structural homology with other MRGPRs, characterized by a seven-transmembrane domain and extracellular N-terminal regions involved in ligand binding. While its exact physiological roles remain under investigation, MRGPRF is implicated in neuroimmune communication, modulating processes like itch sensation, pain signaling, and mast cell activation.
Recombinant human MRGPRF protein is engineered using heterologous expression systems (e.g., HEK293 or insect cells) to produce purified, functional receptor proteins for research. This allows scientists to study MRGPRF's ligand interactions, signaling pathways (e.g., Gαq/11 or β-arrestin), and downstream effects in vitro. The recombinant form often includes tags (e.g., FLAG or His-tag) for detection and purification.
Current research focuses on MRGPRF's potential as a therapeutic target for neuroinflammatory disorders, chronic pain, or allergic diseases. Challenges include clarifying its endogenous ligands and tissue-specific functions. Advances in structural biology (e.g., cryo-EM) and transgenic models continue to unravel MRGPRF's role in health and disease, driving interest in its pharmacological modulation.
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