| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOGAT1 |
| Uniprot No | Q96PD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-334 aa |
| 活性数据 | MKVEFAPLNIQLARRLQTVAVLQWVLSFLTGPMSIGITVMLIIHNYLFLYIPYLMWLYFDWHTPERGGRRSSWIKNWTLWKHFKDYFPIHLIKTQDLDPSHNYIFGFHPHGIMAVGAFGNFSVNYSDFKDLFPGFTSYLHVLPLWFWCPVFREYVMSVGLVSVSKKSVSYMVSKEGGGNISVIVLGGAKESLDAHPGKFTLFIRQRKGFVKIALTHGASLVPVVSFGENELFKQTDNPEGSWIRTVQNKLQKIMGFALPLFHARGVFQYNFGLMTYRKAIHTVVGRPIPVRQTLNPTQEQIEELHQTYMEELRKLFEEHKGKYGIPEHETLVLK |
| 分子量 | 63.69 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MOGAT1蛋白的参考文献示例(部分内容基于虚构整合，仅作格式参考)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Human MOGAT1 in Insect Cells"
**作者**: Lee S et al.
**摘要**: 本文报道了利用杆状病毒表达系统在昆虫细胞中高效表达重组人MOGAT1蛋白，并通过体外酶活实验证实其催化单酰基甘油(MAG)转化为二酰基甘油(DAG)的功能，为研究脂代谢调控机制提供工具。
2. **文献名称**: "Structural Insights into Human MOGAT1: A Key Enzyme in Triglyceride Biosynthesis"
**作者**: Zhang Y et al.
**摘要**: 通过X射线晶体学解析了重组人MOGAT1蛋白的三维结构，揭示了其底物结合域的关键氨基酸残基，并探讨了其与肥胖相关疾病的潜在联系。
3. **文献名称**: "Role of MOGAT1 in Hepatic Lipid Accumulation: Studies Using Recombinant Protein and Knockout Models"
**作者**: Tanaka K et al.
**摘要**: 结合重组MOGAT1蛋白的体外功能实验与小鼠模型，证明MOGAT1在肝脏甘油三酯合成中起关键作用，其过表达可能导致非酒精性脂肪肝(NAFLD)的发展。
4. **文献名称**: "Development of a High-Throughput Assay for MOGAT1 Activity Using Purified Recombinant Protein"
**作者**: Gupta R et al.
**摘要**: 建立了一种基于重组MOGAT1蛋白的高通量酶活检测方法，用于筛选小分子抑制剂，为代谢性疾病药物开发提供平台。
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**注**：以上文献为示例性质，实际研究中请通过PubMed或Web of Science等数据库检索关键词"MOGAT1"、"recombinant protein"等获取最新研究。真实文献可能集中于MOGAT家族功能或疾病关联，针对重组人源蛋白的纯化与功能研究相对较少。
**Background of Recombinant Human MOGAT1 Protein**
Monoglyceride acyltransferase 1 (MOGAT1), also known as MGAT1. is a key enzyme in lipid metabolism, belonging to the acyltransferase family. It catalyzes the conversion of monoacylglycerol (MAG) to diacylglycerol (DAG), a critical step in triglyceride (TG) synthesis within the glycerol phosphate pathway. Primarily expressed in tissues with high lipid turnover—such as the small intestine, liver, and adipose tissue—MOGAT1 plays a vital role in dietary fat absorption and energy storage. By facilitating DAG formation, it enables subsequent TG synthesis, essential for packaging dietary lipids into chylomicrons or storing energy in lipid droplets.
Dysregulation of MOGAT1 activity is implicated in metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Its intestinal expression highlights its importance in postprandial lipid metabolism, making it a potential therapeutic target for managing obesity-related conditions. Recombinant human MOGAT1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and structural integrity, enabling biochemical studies, inhibitor screening, and mechanistic exploration of lipid metabolism. Research leveraging this recombinant protein contributes to understanding its physiological roles and developing interventions for metabolic diseases linked to aberrant lipid handling.
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