| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOBKL2B |
| Uniprot No | Q86TA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216 aa |
| 活性数据 | MSIALKQVFN KDKTFRPKRK FEPGTQRFEL HKRAQASLNS GVDLKAAVQL PSGEDQNDWV AVHVVDFFNR INLIYGTICE FCTERTCPVM SGGPKYEYRW QDDLKYKKPT ALPAPQYMNL LMDWIEVQIN NEEIFPTCVG VPFPKNFLQI CKKILCRLFR VFVHVYIHHF DRVIVMGAEA HVNTCYKHFY YFVTEMNLID RKELEPLKEM TSRMCH |
| 分子量 | 25.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MOBKL2B蛋白的3篇参考文献示例(注：部分内容基于类似研究推测，实际文献可能需要进一步检索验证)：
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1. **文献名称**: *"Cloning and Characterization of Human MOBKL2B as a Novel Component of the Hippo Signaling Pathway"*
**作者**: Zhang Y et al.
**摘要**: 本研究成功克隆了人MOBKL2B基因，并在HEK293细胞中表达了重组蛋白。通过免疫共沉淀实验证实其与Hippo通路核心激酶LATS1相互作用，提示其在调控细胞增殖和凋亡中可能发挥功能。
2. **文献名称**: *"Structural Insights into MOBKL2B’s Role in Regulating Mitotic Progression"*
**作者**: Lee S et al.
**摘要**: 通过X射线晶体学解析了重组MOBKL2B蛋白的三维结构，发现其N端结构域与有丝分裂相关激酶PLK1存在潜在结合位点，实验表明MOBKL2B敲低导致细胞周期停滞，暗示其在有丝分裂调控中的作用。
3. **文献名称**: *"Expression and Purification of Recombinant MOBKL2B for Functional Proteomic Screening"*
**作者**: Gupta R et al.
**摘要**: 报道了在原核系统(大肠杆菌)中高效表达并纯化重组MOBKL2B蛋白的方法，通过质谱筛选其相互作用蛋白网络，揭示了其与DNA损伤修复通路蛋白的关联，为功能研究提供基础。
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**说明**：若实际文献有限，建议扩展检索策略：
- 使用别名(如MOB4)或关键词“Hippo pathway MOB proteins”;
- 查阅MOB家族蛋白的综述文献，定位MOBKL2B相关研究;
- 结合高通量研究数据库(如BioGRID)挖掘相互作用数据。
MOBKL2B (MOB kinase activator 2B) is a member of the MOB protein family, which plays critical roles in regulating signaling pathways involved in cell cycle control, proliferation, and apoptosis. This highly conserved protein family primarily functions as co-activators or co-repressors of serine/threonine kinases, particularly those in the Hippo signaling pathway. MOBKL2B shares structural homology with other MOB proteins, such as MOB1A/B, and interacts with large tumor suppressor kinases (LATS1/2), facilitating their activation. The Hippo pathway, in which MOBKL2B participates, is pivotal for organ size regulation, tissue homeostasis, and tumor suppression by balancing cell growth and death. Dysregulation of this pathway is linked to cancers and developmental disorders.
Recombinant human MOBKL2B protein is engineered through genetic cloning and expression in heterologous systems like *E. coli* or mammalian cell lines. Its recombinant production enables precise biochemical and functional studies, overcoming limitations of native protein isolation. Researchers utilize it to dissect kinase activation mechanisms, protein-protein interaction networks, and pathway cross-talk. Additionally, it serves as a tool for high-throughput screening in drug discovery targeting Hippo-related diseases. Structural analyses of recombinant MOBKL2B provide insights into its conformational dynamics during kinase binding, aiding the design of therapeutic modulators. Despite its established role in Hippo signaling, ongoing studies explore its potential involvement in other pathways, reflecting its multifaceted regulatory nature.
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