| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOBP |
| Uniprot No | Q13875 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-81 aa |
| 活性数据 | MSQKPAKEGPRLSKNQKYSEHFSIHCCPPFTFLNSKKEIVDRKYSICKSGCFYQKKEEDWICCACQKTRLKRKIRPTPKKK |
| 分子量 | 34.65 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MOBP蛋白的3篇参考文献概览：
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1. **文献名称**：*Expression and Purification of Recombinant Human Myelin Oligodendrocyte Basic Protein (MOBP) in Escherichia coli*
**作者**：Smith J. et al. (1995)
**摘要**：研究利用大肠杆菌系统成功表达并纯化重组MOBP蛋白，通过亲和层析和离子交换色谱获得高纯度产物，抗体结合实验证实其免疫原性，为后续研究提供工具。
2. **文献名称**：*Structural Characterization of MOBP Using X-ray Crystallography: Implications for Myelin Stability*
**作者**：Lee H. et al. (2010)
**摘要**：通过X射线晶体学解析重组MOBP的C端结构域，揭示其与脂膜结合的分子机制，提出其在维持髓鞘结构中的作用，为神经系统疾病研究提供结构基础。
3. **文献名称**：*Recombinant MOBP-induced Experimental Autoimmune Encephalomyelitis in Rodents: A Multiple Sclerosis Model*
**作者**：Zhang Y. et al. (2018)
**摘要**：利用重组MOBP蛋白诱导小鼠模型，模拟多发性硬化症的自身免疫反应，发现其触发T细胞反应及脱髓鞘病理，支持MOBP作为该疾病潜在抗原的假说。
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**备注**：上述文献为示例，实际研究中建议通过PubMed、Web of Science等平台结合关键词检索(如“recombinant MOBP”、“myelin protein expression”)获取最新数据。
Myelin-associated Oligodendrocyte Basic Protein (MOBP) is a crucial component of the central nervous system (CNS), primarily expressed in oligodendrocytes. It plays a vital role in the formation, stability, and maintenance of myelin sheaths, which insulate axons to ensure efficient nerve signal transmission. MOBP is localized to the dense cytosolic regions of myelin and interacts with other structural proteins, such as myelin basic protein (MBP), contributing to the compact multilayered architecture of myelin. Structurally, it contains multiple hydrophobic domains and undergoes post-translational modifications, including phosphorylation, which may regulate its function.
Genetic studies link MOBP variants to neurodegenerative disorders like multiple sclerosis (MS), where demyelination and immune-mediated damage occur. Elevated anti-MOBP autoantibodies have been detected in some MS patients, suggesting its involvement in autoimmune pathogenesis. Additionally, MOBP expression alterations are associated with psychiatric conditions, including schizophrenia, though mechanisms remain unclear.
Recombinant MOBP protein, produced via bacterial or mammalian expression systems, enables in vitro studies to explore its biological roles and disease associations. It serves as an antigen for antibody development, a tool for elucidating myelin dynamics, and a potential therapeutic target. Research on recombinant MOBP enhances understanding of myelin biology and CNS disorders, bridging molecular insights to clinical applications.
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