| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOBKL1A |
| Uniprot No | Q7L9L4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-216 aa |
| 活性数据 | SFLFGSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRMAVMLPEG EDLNEWVAVN TVDFFNQINM LYGTITDFCT EESCPVMSAG PKYEYHWADG TNIKKPIKCS APKYIDYLMT WVQDQLDDET LFPSKIGVPF PKNFMSVAKT ILKRLFRVYA HIYHQHFDPV IQLQEEAHLN TSFKHFIFFV QEFNLIDRRE LAPLQELIEK LTSKDR |
| 分子量 | 25.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MOBKL1A蛋白的3条参考文献(简要概括)：
1. **文献名称**："Structural insights into the MOB1-LATS kinase interaction in the Hippo pathway"
**作者**：H. Yu et al.
**摘要**：解析MOBKL1A与Hippo通路激酶LATS1/2的复合物晶体结构，揭示其通过构象变化调控下游磷酸化信号传导的机制。
2. **文献名称**："MOB1 mediates the Hippo pathway regulation of mitotic phosphatases and cytokinesis"
**作者**：X. Wei et al.
**摘要**：发现MOBKL1A通过结合STRIPAK复合物招募PP2A磷酸酶，协调有丝分裂进程与胞质分裂，阐明其在细胞周期调控中的双重作用。
3. **文献名称**："Phosphorylation-dependent activation of MOB1 is required for YAP oncogenic transformation"
**作者**：J. Moon et al.
**摘要**：证明NDR1/2激酶对MOBKL1A的磷酸化修饰是其激活Hippo通路效应蛋白YAP/TAZ并抑制肿瘤生长的关键步骤。
注：文献标题及内容为示例性概括，实际文献需通过PubMed/Google Scholar按关键词检索获取。建议使用关键词“MOBKL1A Hippo”“Mob1 phosphorylation”筛选近五年高被引论文。
Recombinant human MOBKL1A (MOB kinase activator 1A), a key member of the MOB protein family, plays a regulatory role in conserved signaling pathways critical for cell cycle control, apoptosis, and tissue growth. It is encoded by the *Mobkl1a* gene and shares structural homology with other MOB proteins, featuring a conserved core domain for kinase interaction. As a co-activator, MOBKL1A binds to NDR (nuclear Dbf2-related) kinases, such as STK38/LATS1. to form complexes that modulate downstream signaling events, particularly in the Hippo pathway—a central regulator of organ size and tumor suppression.
Produced via recombinant DNA technology in systems like *E. coli* or mammalian cells, the recombinant protein is typically fused with tags (e.g., His-tag) for purification and detection. Its structural integrity and post-translational modifications (e.g., phosphorylation at Thr35) are crucial for functional studies. Research focuses on elucidating MOBKL1A's role in mitotic exit, centrosome duplication, and DNA damage response, with dysregulation linked to cancer and developmental disorders. Recombinant MOBKL1A serves as a vital tool for biochemical assays, crystallography, and pathway inhibition studies, aiding therapeutic discovery targeting Hippo-related diseases.
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