| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MNAT1 |
| Uniprot No | P51948 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK LEEALYEYQP LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA FSGLFWQPS |
| 分子量 | 35.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural Insights into the MNAT1-CDK7-CycH Complex in Human Transcription Regulation"**
- Author: Liu et al. (2020)
- 摘要：通过冷冻电镜解析人MNAT1与CDK7、CycH复合物的三维结构，揭示其在RNA聚合酶II磷酸化和转录启动中的调控机制，重组MNAT1蛋白在此过程中展现关键构象变化。
2. **"Recombinant MNAT1 Facilitates DNA Repair via Interaction with XPA in Nucleotide Excision Repair"**
- Author: Tanaka et al. (2018)
- 摘要：研究表明重组MNAT1蛋白直接结合XPA，增强核苷酸切除修复效率，为MNAT1在DNA损伤应答中的功能提供生化证据。
3. **"MNAT1 Stabilizes CDK7 to Promote Cell Cycle Progression and Cancer Cell Proliferation"**
- Author: Rodriguez et al. (2021)
- 摘要：利用重组MNAT1验证其与CDK7的结合，证明复合物通过维持CDK7稳定性驱动细胞周期G1/S转换，靶向该互作可抑制肿瘤生长。
4. **"Expression and Purification of Functional Human MNAT1 for Kinase Activity Assays"**
- Author: Chen & Wang (2019)
- 摘要：报道重组MNAT1蛋白在大肠杆菌中的可溶性表达优化方案，证明其体外协同CDK7激酶活性，为相关药物筛选提供工具。
(注：以上为模拟文献，实际文献需通过PubMed、Google Scholar等平台检索确认。)
MNAT1 (menin-MAML complex subunit-associated protein 1), also known as CDK-activating kinase assembly factor MAT1. is a critical regulatory protein involved in cell cycle progression, transcriptional regulation, and chromatin modification. It functions as a component of the CDK-activating kinase (CAK) complex, which includes CDK7. cyclin H, and MNAT1. This complex plays a dual role: it regulates the activity of RNA polymerase II by phosphorylating its C-terminal domain (CTD) during transcription initiation, and it activates cyclin-dependent kinases (CDKs) essential for cell cycle transitions, particularly G1/S and G2/M checkpoints.
MNAT1 acts as an assembly scaffold, stabilizing interactions between CDK7 and cyclin H, thereby enhancing CAK kinase activity. Its structural domains, including a conserved RING finger and a hydrophobic region, mediate protein-protein interactions critical for these functions. Beyond transcription and cell cycle control, MNAT1 is implicated in DNA repair and apoptosis, interacting with pathways like transcription-coupled repair (TCR).
Dysregulation of MNAT1 has been linked to cancers, as its overexpression may promote tumorigenesis by disrupting cell cycle checkpoints or transcriptional fidelity. Recombinant human MNAT1 protein is widely used to study its biochemical properties, CAK complex assembly mechanisms, and therapeutic targeting in diseases like cancer. Its role in coordinating transcription with cell cycle progression makes it a key focus in molecular biology and oncology research.
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