| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAST3 |
| Uniprot No | O60307 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-723aa |
| 活性数据 | MDESSLLRRRGLQKELSLPRRGRGCRSGNRKSLVVGTPSPTLSRPLSPLS VPTAGSSPLDSPRNFSAASALNFPFARRADGRRWSLASLPSSGYGTNTPS STLSSSSSSRERLHQLPFQPTPDELHFLSKHFRSSENVLDEEGGRSPRLR PRSRSLSPGRATGTFDNEIVMMNHVYRERFPKATAQMEGRLQEFLTAYAP GARLALADGVLGFIHHQIVELARDCLAKSGENLVTSRYFLEMQEKLERLL QDAHERSDSEEVSFIVQLVRKLLIIISRPARLLECLEFDPEEFYHLLEAA EGHAREGQGIKTDLPQYIIGQLGLAKDPLEEMVPLSHLEEEQPPAPESPE SRALVGQSRRKPCESDFETIKLISNGAYGAVYLVRHRDTRQRFAIKKINK QNLILRNQIQQVFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGG DCATLLKNMGPLPVDMARLYFAETVLALEYLHNYGIVHRDLKPDNLLITS LGHIKLTDFGLSKIGLMSMATNLYEGHIEKDAREFIDKQVCGTPEYIAPE VIFRQGYGKPVDWWAMGVVLYEFLVGCVPFFGDTPEELFGQVVSDEIMWP EGDEALPADAQDLITRLLRQSPLDRLGTGGTHEVKQHPFFLALDWAGLLR HKAEFVPQLEAEDDTSYFDTRSERYRHLGSEDDETNDEESSTEIPQFSSC SHRFSKVYSSSEFLAVQPTPTFA |
| 分子量 | 130 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAST3蛋白的3篇示例参考文献（注：部分内容基于研究领域常见方向模拟，若需真实文献请提供更具体信息）：
1. **文献名称**：*"Expression and functional characterization of recombinant human MAST3 kinase in neuronal signaling"*
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人MAST3的激酶结构域，并验证其磷酸化底物TAO2的能力，提示其在神经元微管相关信号通路中的调控作用。
2. **文献名称**：*"Structural insights into MAST3 kinase domain and its autoinhibitory mechanism"*
**作者**：Rodríguez S, et al.
**摘要**：通过X射线晶体学解析重组MAST3激酶结构域的三维结构，揭示其自抑制构象，为靶向MAST3的药物设计提供依据。
3. **文献名称**：*"MAST3 modulates NF-κB activation through phosphorylation of TRAF6 in inflammatory responses"*
**作者**：Wang Y, et al.
**摘要**：利用昆虫细胞表达系统获得全长的重组人MAST3蛋白，证明其通过磷酸化TRAF6增强NF-κB通路活性，参与炎症反应调控。
如需真实文献，建议在PubMed或Google Scholar中检索关键词：**"recombinant MAST3"、"MAST3 kinase"**，并筛选近年发表的蛋白质功能或结构研究。
Recombinant human MAST3 (Microtubule-Associated Serine/Threonine-Protein Kinase 3) is a engineered form of the MAST3 protein, a member of the MAST kinase family characterized by a conserved kinase domain and a PDZ domain. MAST3 plays roles in cellular signaling, cytoskeletal regulation, and neuronal development. It interacts with various partners, including the tumor suppressor PTEN, to modulate pathways like PI3K/AKT, which are critical for cell survival, growth, and synaptic function. Dysregulation of MAST3 has been implicated in neurological disorders, cancers, and developmental defects.
Produced via recombinant DNA technology in systems like E. coli or mammalian cells, the recombinant MAST3 protein retains its enzymatic activity and structural integrity, enabling functional studies. Researchers use it to dissect its kinase activity, substrate specificity, and regulatory mechanisms. Its PDZ domain-mediated protein interactions are of particular interest in understanding signaling networks and disease pathways. Additionally, recombinant MAST3 serves as a tool for screening potential inhibitors or modulators in drug discovery, especially for conditions linked to MAST3 dysfunction. Its applications extend to structural biology, aiding in X-ray crystallography or cryo-EM studies to resolve mechanistic details. This recombinant protein is pivotal in bridging molecular insights with therapeutic innovation.
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