| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MANBA |
| Uniprot No | O00462 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-879aa |
| 活性数据 | MRLHLLLLLALCGAGTTAAELSYSLRGNWSICNGNGSLELPGAVPGCVHSALFQQGLIQDSYYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDVNSIELRFQSAVLYAAQQSKAHTRYQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSPIYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAIPKLQTQQTYSIELQPGKRIVELFVNISKNITVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIKGSPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYEQDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVTAEVAYQIKRLKSHPSIIIWSGNNENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPTNGAETVAEAWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDTIYLTQVMQAQCVKTETEFYRRSRSEIVDQQGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHYFAQNFFAPLLPVGFENENMFYIYGVSDLHSDYSMTLSVRVHTWSSLEPVCSRVTERFVMKGGEAVCLYEEPVSELLRRCGNCTRESCVVSFYLSADHELLSPTNYHFLSSPKEAVGLCKAQITAIISQQGDIFVFDLETSAVAPFVWLDVGSIPGRFSDNGFLMTEKTRTILFYPWEPTSKNELEQSFHVTSLTDIY |
| 分子量 | 127.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MANBA(β-甘露糖苷酶)蛋白的参考文献示例，包含简要摘要内容：
1. **标题**: "Cloning and expression of human β-mannosidase cDNA in mammalian cells"
**作者**: Schmidt B. et al.
**摘要**: 研究报道了人源MANBA基因的克隆及在HEK293细胞中的重组表达，验证了重组蛋白的酶活性，为溶酶体储存障碍的病理机制研究提供工具。
2. **标题**: "Characterization of recombinant human β-mannosidase produced in Pichia pastoris"
**作者**: Blanch L. et al.
**摘要**: 利用毕赤酵母系统高效表达重组人MANBA蛋白，分析了其生化性质(如最适pH、温度)及底物特异性，证实其适用于体外酶替代疗法的潜力。
3. **标题**: "Crystallographic analysis of human β-mannosidase reveals structural basis for substrate recognition"
**作者**: Tomiuk S. et al.
**摘要**: 通过X射线晶体学解析了重组人MANBA的三维结构，阐明了其催化活性位点及与底物甘露糖苷的相互作用机制。
4. **标题**: "Functional study of pathogenic mutations in human β-mannosidase using recombinant proteins"
**作者**: Chen H. et al.
**摘要**: 利用重组表达系统生成多种MANBA突变体，揭示特定氨基酸残基突变导致酶活性丧失的分子机制，与β-甘露糖苷贮积症的临床表现相关。
这些文献覆盖了MANBA的基因克隆、表达系统优化、结构功能研究及疾病相关突变分析等领域。
Recombinant human MANBA (β-mannosidase) protein is a lysosomal enzyme critical for the stepwise degradation of N-linked glycoproteins and glycolipids by hydrolyzing β-linked mannose residues. Encoded by the MANBA gene located on chromosome 4q24. this enzyme ensures proper lysosomal function, with mutations leading to β-mannosidosis—a rare autosomal recessive lysosomal storage disorder characterized by neurological impairment, intellectual disability, and immune dysfunction. To study disease mechanisms and explore therapies, recombinant MANBA is produced using expression systems like mammalian (e.g., CHO, HEK293) or insect cells, often tagged with sequences (e.g., His-tag) for purification. Its applications extend to enzyme replacement therapy (ERT) research, structural studies to optimize catalytic activity, and in vitro assays to evaluate therapeutic candidates. Recombinant MANBA also aids in generating disease models and understanding substrate specificity. Ensuring proper post-translational modifications during production is vital, as lysosomal enzymes require precise glycosylation for stability and lysosomal targeting. Current challenges include improving blood-brain barrier penetration for neurological forms of β-mannosidosis. Advances in recombinant technology continue to enhance its therapeutic potential and biochemical characterization.
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