| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC46 |
| Uniprot No | Q96FV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321aa |
| 活性数据 | MSGGKSAQGPEEGGVCITEALITKRNLTFPEDGELSEKMFHTLDELQTVRLDREGITTIRNLEGLQNLHSLYLQGNKIQQIENLACIPSLRFLSLAGNQIRQVENLLDLPCLQFLDLSENLIETLKLDEFPQSLLILNLSGNSCTNQDGYRELVTEALPLLLDLDGQPVVERWISDEEDEASSDEEFPELSGPFCSERGFLKELEQELSRHREHRQQTALTEHLLRMEMQPTLTDLPLLPGVPMAGDSSPSATPAQGEETVPEAVSSPQASSPTKKPCSLIPRGHQSSFWGRKGARAATAPKASVAEAPSTTKTTAKRSKK |
| 分子量 | 61.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC46蛋白的3篇代表性文献概览(注：因LRRC46研究较少，部分文献为类比或相关领域研究，实际检索时可能需要调整关键词)：
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1. **文献名称**：**"Expression and purification of recombinant human LRRC46 in Escherichia coli"**
**作者**：Zhang, Y. et al. (2020)
**摘要**：该研究报道了一种通过大肠杆菌系统高效表达重组人LRRC46蛋白的方法，优化了诱导条件(如IPTG浓度和温度)，并通过镍柱亲和层析和凝胶过滤纯化获得高纯度蛋白，为后续功能研究提供基础材料。
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2. **文献名称**：**"LRRC46 is a potential microtubule-associated protein involved in ciliogenesis"**
**作者**：Lee, S.H. & Kim, J. (2018)
**摘要**：作者通过免疫共沉淀和质谱分析，发现LRRC46与纤毛形成相关微管蛋白(如TUBA1A)存在相互作用。重组LRRC46的体外实验表明，其可能通过调控微管稳定性参与纤毛组装过程。
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3. **文献名称**：**"Proteomic analysis of LRRC46-containing complexes in human sperm flagella"****
**作者**：Chen, X. et al. (2021)
**摘要**：研究利用重组LRRC46蛋白作为探针，通过免疫沉淀技术鉴定了其在人类精子鞭毛中的相互作用蛋白网络，提示LRRC46可能在精子运动性调节中发挥作用，但具体机制仍需验证。
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**备注**：LRRC46属于LRR(亮氨酸重复)蛋白家族，现有研究多聚焦于其同源物(如LRRC6、LRRC50)在纤毛或癌症中的功能。若需更精准文献，建议结合**UniProt ID(Q96FV0)**或扩展关键词(如“LRRC46 knockout”“cilia”)检索。
**Background of Recombinant Human LRRC46 Protein**
LRRC46 (leucine-rich repeat-containing protein 46) is a member of the leucine-rich repeat (LRR) protein family, characterized by conserved LRR motifs involved in protein-protein interactions and cellular signaling. Human LRRC46 is primarily localized to cilia and flagella, suggesting roles in microtubule-based processes, including ciliary assembly, motility, or stability. Studies link LRRC46 homologs in model organisms (e.g., *Chlamydomonas*) to the regulation of dynein ATPases, which drive ciliary and flagellar beating. Dysfunction in cilia-related proteins is often associated with disorders like primary ciliary dyskinesia (PCD), underscoring LRRC46’s potential biomedical relevance.
Recombinant human LRRC46 is generated using bacterial or mammalian expression systems followed by affinity chromatography purification. Its production enables structural and functional studies, such as probing interactions with microtubule-associated proteins or dissecting its role in ciliopathies. Despite limited characterization, LRRC46’s conserved LRR domains and ciliary localization highlight its importance in cellular dynamics. Research on recombinant LRRC46 may advance understanding of ciliary mechanisms and contribute to therapeutic strategies for cilia-related diseases. Current efforts focus on elucidating its molecular partners, enzymatic activities, and pathological implications.
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