| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC42 |
| Uniprot No | Q9Y546 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 活性数据 | MSYYLSSENHLDPGPIYMRENGQLHMVNLALDGVRSSLQKPRPFRLFPKGFSVELCMNREDDTARKEKTDHFIFTYTREGNLRYSAKSLFSLVLGFISDNVDHIDSLIGFPEQIAEKLFSAAEARQKFTEPGAGLRALQKFTEAYGSLVLCSLCLRNRYLVISEKLEEIKSFRELTCLDLSCCKLGDEHELLEHLTNEALSSVTQLHLKDNCLSDAGVRKMTAPVRVMKRGLENLTLLDLSCNPEITDAGIGYLFSFRKLNCLDISGTGLKDIKTVKHKLQTHIGLVHSKVPLKEFDHSNCKTEGWADQIVLQWERVTAEAVKPRETSEPRAAAQRFYGKRSRAEAPLKCPLADTHMNSSEKLQFYKEKAPDCHGPVLKHEAISSQESKKSKKRPFEESETEQNNSSQPSKQKYVCLAVEDWDLLNSY |
| 分子量 | 75 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC42蛋白的3篇参考文献(示例为虚构内容，实际文献需通过学术数据库查询)：
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1. **文献名称**：*LRRC42 regulates ciliogenesis by interacting with microtubule dynamics*
**作者**：Smith J, Lee K, et al.
**摘要**：本研究揭示了LRRC42通过结合微管蛋白调控纤毛生成，重组人LRRC42蛋白在体外实验中显示促进微管稳定性，并影响纤毛相关疾病模型的功能修复。
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2. **文献名称**：*Expression and purification of recombinant human LRRC42 in E. coli for structural analysis*
**作者**：Chen X, Wang Y, et al.
**摘要**：报道了一种利用大肠杆菌表达系统高效制备重组人LRRC42蛋白的方法，并通过质谱和X射线晶体学解析其结构，发现其LRR结构域可能参与蛋白质相互作用。
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3. **文献名称**：*LRRC42 deficiency disrupts spermatogenesis via dysregulated cell cycle progression*
**作者**：Zhang R, Tanaka H, et al.
**摘要**：研究表明，LRRC42基因缺失导致小鼠精子发生异常，重组蛋白回补实验证实其通过调控细胞周期蛋白Cyclin B1影响生殖细胞分裂，为男性不育机制提供新见解。
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**注**：以上为模拟示例，实际文献建议通过PubMed、Google Scholar等平台以关键词“recombinant human LRRC42”或“LRRC42 protein function”检索。若需具体文献，请提供更多研究背景以便精准推荐。
**Background of Recombinant Human LRRC42 Protein**
Recombinant human LRRC42 (Leucine-Rich Repeat-Containing Protein 42) is a engineered protein derived from the LRRC42 gene, which encodes a member of the leucine-rich repeat (LRR) protein family. LRR domains are evolutionarily conserved structural motifs involved in protein-protein interactions, cellular signaling, and molecular recognition. LRRC42. specifically, is hypothesized to play roles in cytoskeletal organization, cilia-related functions, or cell cycle regulation, though its precise biological mechanisms remain incompletely characterized.
Studies suggest LRRC42 may interact with microtubules or other cytoskeletal components, potentially influencing cell division, motility, or intracellular trafficking. Its expression has been detected in various tissues, with emerging links to ciliopathies—genetic disorders affecting cilia structure/function—and diseases like polycystic kidney disease. However, functional data are limited, necessitating further research to clarify its physiological and pathological relevance.
Recombinant LRRC42 is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His-tag) for purification and detection. This tool enables in vitro studies, such as binding assays, structural analysis (e.g., crystallography), or antibody development. It also aids in exploring LRRC42's interactome, post-translational modifications, and potential therapeutic targeting. Current efforts focus on elucidating its role in cellular homeostasis and disease pathways, bridging gaps between its molecular features and functional impact.
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