| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC45 |
| Uniprot No | Q96CN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-670aa |
| 活性数据 | MEEFRRSYSRLCRESGAEPQEAVLQQLHQLPRGRLDLATQSLTVETCRALGKLLPRETLCTELVLSDCMLSEEGATLLLRGLCANTVLRFLDLKGNNLRAAGAEALGKLLQQNKSIQSLTLEWNSLGTWDDAFATFCGGLAANGALQRLDLRNNQISHKGAEELALALKGNTTLQQLDLRWNNVGLLGGRALMNCLPSNRTLWRLDLAGNNIPGDVLRAVEQAMGHSQDRLTTFQENQARTHVLSKEVQHLREEKSKQFLDLMETIDKQREEMAKSSRASAARVGQLQEALNERHSIINALKAKLQMTEAALALSEQKAQDLGELLATAEQEQLSLSQRQAKELKLEQQEAAERESKLLRDLSAANEKNLLLQNQVDELERKFRCQQEQLFQTRQEMTSMSAELKMRAIQAEERLDMEKRRCRQSLEDSESLRIKEVEHMTRHLEESEKAMQERVQRLEAARLSLEEELSRVKAAALSERGQAEEELIKAKSQARLEEQQRLAHLEDKLRLLAQARDEAQGACLQQKQVVAEAQTRVSQLGLQVEGLRRRLEELQQELSLKDQERVAEVSRVRVELQEQNGRLQAELAAQEALREKAAALERQLKVMASDHREALLDRESENASLREKLRLREAEIARIRDEEAQRASFLQNAVLAYVQASPVRTLSPPK |
| 分子量 | 102.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC45蛋白的**模拟参考文献示例**(由于该蛋白研究较少，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Cloning and Functional Characterization of Human LRRC45 in Ciliogenesis"*
**作者**：Chen X, et al.
**摘要**：本研究通过构建重组人LRRC45蛋白，发现其可与CEP164相互作用，调控纤毛组装过程，并在HeLa细胞中验证了其缺失导致的纤毛形成障碍。
2. **文献名称**：*"Expression and Purification of Recombinant LRRC45 in Mammalian Cells for Structural Analysis"*
**作者**：Tanaka K, et al.
**摘要**：报道了在HEK293细胞中表达带有FLAG标签的重组人LRRC45蛋白，通过亲和层析纯化获得高纯度蛋白，并利用冷冻电镜初步解析其结构域构象。
3. **文献名称**：*"LRRC45 as a Novel Binding Partner of Dynein in Cell Cycle Progression"*
**作者**：Wang L, et al.
**摘要**：通过酵母双杂交筛选发现LRRC45与细胞质动力蛋白复合体结合，重组蛋白体外实验证实其参与调控有丝分裂中微管运动功能。
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**注意**：以上为假设性示例，实际文献需在**PubMed**或**Google Scholar**等平台检索关键词(如“LRRC45 recombinant”“LRRC45 function”)。若研究较新，建议补充相关领域综述或预印本数据库(如bioRxiv)资源。
The leucine-rich repeat-containing protein 45 (LRRC45) is a relatively understudied member of the LRR protein family, characterized by repeats of 20–30 amino acid motifs rich in leucine residues. These proteins typically mediate protein-protein interactions, playing roles in signaling, structural organization, and cellular processes. LRRC45 is evolutionarily conserved and expressed in various tissues, with notable presence in ciliated cells. Structural studies suggest it forms coiled-coil domains, potentially linking it to cytoskeletal or ciliary functions.
Recent research highlights LRRC45's association with primary cilia—a sensory organelle critical for developmental signaling and homeostasis. It interacts with proteins involved in ciliogenesis, such as CEP131 and CCDC14. suggesting a role in ciliary basal body anchoring or microtubule organization. Dysregulation of ciliary proteins is linked to ciliopathies, cancers, and neurodevelopmental disorders, implicating LRRC45 in these pathways, though mechanistic details remain unclear.
Recombinant human LRRC45 protein, produced via bacterial or mammalian expression systems, enables biochemical studies (e.g., interaction mapping, antibody development) and functional assays to dissect its role in cilia-related pathways. Its recombinant form is also explored for potential diagnostic or therapeutic applications, particularly in diseases tied to ciliary dysfunction. Despite progress, LRRC45's precise molecular functions and disease relevance require further investigation, positioning it as an emerging target in cell biology and translational research.
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