| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOH11CR2A |
| Uniprot No | O00534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-786aa |
| 活性数据 | MVHFCGLLTLHREPVPLKSISVSVNIYEFVAGVSATLNYENEEKVPLEAFFVFPMDEDSAVYSFEALVDGKKIVAELQDKMKARTNYEKAISQGHQAFLLEGDSSSRDVFSCNVGNLQPGSKAAVTLKYVQELPLEADGALRFVLPAVLNPRYQFSGSSKDSCLNVKTPIVPVEDLPYTLSMVATIDSQHGIEKVQSNCPLSPTEYLGEDKTSAQVSLAAGHKFDRDVELLIYYNEVHTPSVVLEMGMPNMKPGHLMGDPSAMVSFYPNIPEDQPSNTCGEFIFLMDRSGSMQSPMSSQDTSQLRIQAAKETLILLLKSLPIGCYFNIYGFGSSYEACFPESVKYTQQTMEEALGRVKLMQADLGGTEILAPLQNIYRGPSIPGHPLQLFVFTDGEVTDTFSVIKEVRINRQKHRCFSFGIGEGTSTSLIKGIARASGGTSEFITGKDRMQSKALRTLKRSLQPVVEDVSLSWHLPPGLSAKMLSPEQTVIFRGQRLISYAQLTGRMPAAETTGEVCLKYTLQGKTFEDKVTFPLQPKPDVNLTIHRLAAKSLLQTKDMGLRETPASDKKDALNLSLESGVISSFTAFIAINKELNKPVQGPLAHRDVPRPILLGASAPLKIKCQSGFRKALHSDRPPSASQPRGELMCYKAKTFQMDDYSLCGLISHKDQHSPGFGENHLVQLIYHQNANGSWDLNEDLAKILGMSLEEIMAAQPAELVDSSGWATILAVIWLHSNGKDLKCEWELLERKAVAWMRAHAGSTMPSVVKAAITFLKSSVDPAIFAF |
| 分子量 | 112.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LOH11CR2A蛋白的模拟参考文献示例(仅供学术参考，需根据实际文献调整)：
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1. **《重组人LOH11CR2A蛋白的克隆表达与功能初探》**
**作者：Zhang et al.**
**摘要**：本研究通过PCR扩增LOH11CR2A基因，成功构建原核表达载体，并利用大肠杆菌系统表达纯化重组蛋白。实验证实该蛋白具有抑制肿瘤细胞增殖活性，可能通过调控MAPK信号通路发挥作用。
2. **《LOH11CR2A在卵巢癌细胞中的表观遗传沉默及其临床意义》**
**作者：Wang & Li**
**摘要**：通过甲基化特异性PCR发现LOH11CR2A在卵巢癌组织中高频异常甲基化，导致蛋白表达下调;体外实验显示重组LOH11CR2A可显著诱导癌细胞凋亡，提示其作为肿瘤抑制因子的潜力。
3. **《LOH11CR2A与BRCA1蛋白的相互作用研究》**
**作者：Chen et al.**
**摘要**：采用免疫共沉淀技术证实重组LOH11CR2A蛋白与BRCA1存在直接结合，并参与DNA损伤修复过程。该互作可能解释其在遗传性乳腺癌中的杂合性缺失现象。
4. **《血清LOH11CR2A蛋白作为肺癌早期诊断标志物的验证》**
**作者：Kim et al.**
**摘要**：基于ELISA检测发现肺癌患者血清中LOH11CR2A水平显著低于健康对照，重组蛋白的体外验证实验支持其作为非侵入性诊断标志物的可能性(AUC=0.87)。
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**注**：以上文献为模拟示例，实际研究需通过PubMed、Web of Science等数据库检索真实文献。建议结合关键词“LOH11CR2A recombinant protein”“LOH11CR2A function”等进一步筛选。
LOH11CR2A (Loss of Heterozygosity 11 Chromosomal Region 2A) is a protein encoded by the LOH11CR2A gene located on human chromosome 11q23.3. This genomic region has been frequently associated with cancer due to observed loss of heterozygosity (LOH) in various malignancies, including breast, ovarian, and colorectal cancers, suggesting its potential role as a tumor suppressor. The gene spans approximately 65 kb and encodes a protein composed of 522 amino acids, though alternative splicing produces multiple isoforms. Despite its clinical significance, the precise molecular functions of LOH11CR2A remain under investigation.
Studies indicate that LOH11CR2A may interact with proteins involved in DNA repair, apoptosis, and cell cycle regulation. Its expression is downregulated in certain cancers, correlating with poor prognosis, while overexpression in vitro has been shown to suppress tumor cell proliferation. Recombinant human LOH11CR2A protein is typically produced using bacterial or mammalian expression systems for functional studies. Researchers utilize it to explore its biochemical properties, post-translational modifications, and interactions within signaling pathways. Current efforts focus on elucidating its role in maintaining genomic stability and its potential therapeutic applications in cancer biology. However, further structural and mechanistic studies are required to fully define its contributions to cellular homeostasis and disease pathology.
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