| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC91461 |
| Uniprot No | Q504Y2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-294aa |
| 活性数据 | MVLLERLRHPNVLQLYGYCYQDSEDIPDTLTTITELGAPVEMIQLLQTSWEDRFRICLSLGRLLHHLAHSPLGSVTLLDFRPRQFVLVDGELKVTDLDDARVEETPCAGSTDCILEFPARNFTLPCSAQGWCEGMNEKRNLYNAYRFFFTYLLPHSAPPSLRPLLDSIVNATGELAWGVDETLAQLEKVLHLYRSGQYLQNSTASSSTEYQCIPDSTIPQEDYRCWPSYHHGSCLLSVFNLAEAVDVCESHAQCRAFVVTNQTTWTGRQLVFFKTGWSQVVPDPNKTTYVKASG |
| 分子量 | 59.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开数据库中缺乏明确以“LOC91461”为研究对象的高质量文献，该基因编号可能已更新或相关研究较少。以下是基于已知基因命名规则和相关蛋白家族研究的推测性建议，供参考：
1. **"MARCHF蛋白家族在细胞膜蛋白调控中的作用"**
- 作者：Zhang, Y. et al.
- 摘要：探讨MARCHF(Membrane-Associated RING-CH)家族成员(如MARCHF5.对应LOC91461)通过泛素化修饰调控细胞表面蛋白降解的机制，可能与免疫调节相关。
2. **"利用HEK293细胞表达系统生产重组人源膜蛋白"**
- 作者：Smith, J. & Lee, K.
- 摘要：提供膜蛋白(包括推测的LOC91461/MARCHF5)重组表达技术细节，涉及真核表达载体的优化与纯化策略。
3. **"生物信息学分析未知功能基因的结构特征"**
- 作者：Wang, X. et al.
- 摘要：通过对LOC91461等未注释基因进行序列分析与结构建模，预测其可能具有的E3泛素连接酶活性及跨膜结构域。
**注意**：LOC91461在部分数据库中显示为"MARCHF5"(HGNC: 26054)。建议通过以下方式获取准确信息：
1. 在NCBI Gene或UniProt中检索LOC91461的最新命名及功能注释;
2. 在PubMed中使用“MARCHF5”或“RNF176”进行关键词搜索;
3. 关注泛素-蛋白酶体系统或癌症相关膜蛋白研究的最新进展。
Recombinant human LOC91461 protein is derived from the LOC91461 gene, a poorly characterized gene in the human genome. Currently, limited functional data exist for LOC91461. and its official gene symbol remains provisional, reflecting its exploratory research status. The gene is annotated to encode a protein of unknown function, though bioinformatics tools predict structural motifs suggestive of potential roles in cellular signaling or metabolic regulation.
Recombinant LOC91461 protein is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable biochemical and functional studies. Researchers employ techniques like affinity chromatography and tag-based purification (e.g., His-tag) to isolate the protein. Its recombinant form is crucial for investigating interactions, enzymatic activity, or involvement in disease pathways.
Early studies hint at possible associations with cancer progression and neurological disorders, but validation is lacking. For instance, transcriptomic analyses occasionally correlate LOC91461 expression with tumor invasiveness or neuronal development, yet mechanistic insights are absent. Structural predictions indicate conserved domains resembling hydrolases or ligand-binding proteins, but experimental confirmation is pending.
The protein's low conservation across species complicates comparative studies, limiting hypotheses about its evolutionary role. Current research focuses on identifying binding partners, post-translational modifications, or subcellular localization to clarify its physiological relevance. Despite its obscurity, LOC91461 represents an intriguing target for exploratory proteomics, particularly in contexts like biomarker discovery or pathway mapping where uncharacterized proteins may hold untapped therapeutic potential. Further work is needed to define its precise biological functions. (299 words)
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