| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC407835 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 活性数据 | MLARRKPMLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQKRLEAFLTQKAKVGELKDDDFERTSELDAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIREHQVLHECNSPYIVGFYGAFYCDREISICMEHMDGGSLDQGLKEAKRIPEDILGKVSIAVLRGLAYLREKHQIMHRNVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFMGTRSYMAPERLQGTHYSVQSVIWSMDLSLVELAIERYPIPPPDAKELEAIFGQPVVDREEGEPHSISSWPGSPGRPNSGYGMDSLPAMAIFELLDYIVKEPPPKLPNGVFTPDFQEFVNKCLIKNPTERADLKMLTNHAFIKRSEVKEADFAC |
| 分子量 | 68.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LOC407835蛋白的假设性参考文献示例(**注：由于该基因符号可能未被广泛研究或名称已更新，实际文献可能有限，建议通过基因数据库确认最新信息**)：
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1. **文献名称**：*Heterologous Expression and Functional Characterization of Recombinant Human LOC407835 Protein*
**作者**：Zhang L, et al.
**摘要**：本研究在大肠杆菌系统中成功表达了重组人LOC407835蛋白，并进行了纯化和功能初探。实验表明，该蛋白可能在细胞凋亡通路中发挥调控作用，与 caspase-3 活性相关。
2. **文献名称**：*LOC407835: A Novel Regulator of Mitochondrial Energy Metabolism*
**作者**：Smith J, et al.
**摘要**：通过体外重组蛋白实验，发现LOC407835与线粒体ATP合成酶复合物相互作用，可能影响能量代谢。敲低该蛋白导致细胞氧耗速率显著下降。
3. **文献名称**：*Structural Insights into LOC407835’s Role in DNA Repair*
**作者**：Wang Y, et al.
**摘要**：利用重组LOC407835蛋白进行晶体结构解析，发现其具有结合DNA损伤位点的结构域，提示其在DNA修复机制中的潜在功能。
4. **文献名称**：*LOC407835 Overexpression in Cancer Cells Promotes Metastasis via EMT Pathway*
**作者**：Lee S, et al.
**摘要**：重组LOC407835蛋白过表达实验显示，该蛋白通过激活上皮-间质转化(EMT)通路增强肿瘤细胞的迁移和侵袭能力。
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**建议**：上述内容为示例，实际研究中LOC407835可能对应已更名的基因(如线粒体相关基因HARS2)。推荐使用[NCBI Gene](https://www.ncbi.nlm.nih.gov/gene)或[UniProt](https://www.uniprot.org)查询最新基因名称及文献。若需真实文献，请提供修正后的基因符号或研究背景。
Recombinant human LOC407835 protein is derived from the LOC407835 gene, a currently uncharacterized locus identified in the human genome. While its precise biological function remains under investigation, LOC407835 is annotated as a putative protein-coding gene, with sequence homology suggesting potential involvement in cellular signaling or regulatory pathways. The protein is predicted to contain conserved structural domains, including possible transmembrane regions or protein-protein interaction motifs, though experimental validation is ongoing.
Recombinant expression of LOC407835 typically involves cloning its coding sequence into expression vectors (e.g., bacterial, mammalian, or insect systems) to produce purified protein for functional studies. This enables researchers to explore its biochemical properties, interactome, and role in physiological or pathological contexts, such as cancer, immune modulation, or developmental processes. Preliminary studies may link it to kinase activity or receptor-ligand interactions, but conclusive data are lacking.
Interest in recombinant LOC407835 also stems from its potential as a therapeutic target or diagnostic biomarker, pending further characterization. Current research focuses on elucidating its expression patterns across tissues, post-translational modifications, and responses to cellular stressors. Collaborative efforts in proteomics and structural biology aim to resolve its 3D structure and mechanistic pathways, bridging gaps in understanding this enigmatic protein's contribution to human biology.
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