| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LLGL2 |
| Uniprot No | Q6P1M3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1020aa |
| 活性数据 | MRRFLRPGHDPVRERLKRDLFQFNKTVEHGFPHQPSALGYSPSLRILAIGTRSGAIKLYGAPGVEFMGLHQENNAVTQIHLLPGQCQLVTLLDDNSLHLWSLKVKGGASELQEDESFTLRGPPGAAPSATQITVVLPHSSCELLYLGTESGNVFVVQLPAFRALEDRTISSDAVLQRLPEEARHRRVFEMVEALQEHPRDPNQILIGYSRGLVVIWDLQGSRVLYHFLSSQQLENIWWQRDGRLLVSCHSDGSYCQWPVSSEAQQPEPLRSLVPYGPFPCKAITRILWLTTRQGLPFTIFQGGMPRASYGDRHCISVIHDGQQTAFDFTSRVIGFTVLTEADPAATFDDPYALVVLAEEELVVIDLQTAGWPPVQLPYLASLHCSAITCSHHVSNIPLKLWERIIAAGSRQNAHFSTMEWPIDGGTSLTPAPPQRDLLLTGHEDGTVRFWDASGVCLRLLYKLSTVRVFLTDTDPNENLSAQGEDEWPPLRKVGSFDPYSDDPRLGIQKIFLCKYSGYLAVAGTAGQVLVLELNDEAAEQAVEQVEADLLQDQEGYRWKGHERLAARSGPVRFEPGFQPFVLVQCQPPAVVTSLALHSEWRLVAFGTSHGFGLFDHQQRRQVFVKCTLHPSDQLALEGPLSRVKSLKKSLRQSFRRMRRSRVSSRKRHPAGPPGEAQEGSAKAERPGLQNMELAPVQRKIEARSAEDSFTGFVRTLYFADTYLKDSSRHCPSLWAGTNGGTIYAFSLRVPPAERRMDESVRAEQAKEIQLMHRAPVVGILVLDGHSVPLPEPLEVAHDLSKSPDMQGSHQLLVVSEEQFKVFTLPKVSAKLKLKLTALEGSRVRRVSVAHFGSRRAEDYGEHHLAVLTNLGDIQVVSLPLLKPQVRYSCIRREDVSGIASCVFTKYGQGFYLISPSEFERFSLSTKWLVEPRCLVDSAETKNHRPGNGAGPKKAPSRARNSGTQSDGEEKQPGLVMERALLSDERVLKEIQSTLEGDRGSGNWRSHRAAVGCSLSNGGAE |
| 分子量 | 139.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性参考文献示例，供参考使用(建议通过PubMed或Web of Science获取真实文献)：
1. **文献名称**: "Expression and functional analysis of recombinant human LLGL2 in epithelial polarity regulation"
**作者**: Smith A, et al.
**摘要**: 研究报道了重组人LLGL2蛋白在哺乳动物细胞中的表达与纯化，并发现其通过调控细胞极性相关蛋白(如PAR复合物)参与上皮细胞极性的建立和维持。
2. **文献名称**: "LLGL2 suppresses tumor metastasis by interacting with the SCRIB complex in breast cancer"
**作者**: Chen L, et al.
**摘要**: 利用重组LLGL2蛋白进行体外结合实验，揭示LLGL2通过与SCRIB蛋白结合抑制乳腺癌细胞的侵袭和转移，其缺失导致细胞极性紊乱。
3. **文献名称**: "Structural characterization of human LLGL2 reveals critical domains for membrane association"
**作者**: Tanaka K, et al.
**摘要**: 通过重组表达LLGL2蛋白并进行结构域分析，发现其N端WD40重复结构对细胞膜定位及与磷脂的相互作用至关重要。
4. **文献名称**: "Role of LLGL2 in neuronal development: Insights from recombinant protein rescue experiments"
**作者**: Wang X, et al.
**摘要**: 在神经祖细胞模型中，外源性添加重组人LLGL2蛋白可部分恢复细胞极性和不对称分裂能力，提示其在神经发育中的作用。
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**注意**：以上内容为模拟生成，实际文献需通过学术数据库检索。推荐关键词：*"LLGL2 recombinant"、"LLGL2 cell polarity"、"LLGL2 cancer"*。
Lethal giant larvae homolog 2 (LLGL2) is a conserved scaffolding protein critical for establishing cell polarity, a process governing asymmetric cell division, adhesion, and tissue organization. In humans, LLGL2 regulates epithelial cell polarization by interacting with polarity complexes like the PAR (partitioning defective) network and coordinating cytoskeletal dynamics. It is implicated in vesicle trafficking, cell migration, and maintaining apical-basal polarity in epithelial tissues. Dysregulation of LLGL2 is linked to cancer progression, where its loss disrupts polarity, enhances invasiveness, and correlates with poor prognosis in breast, lung, and colorectal cancers.
Recombinant human LLGL2 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables functional studies to dissect its roles in polarity signaling and disease mechanisms. Purified LLGL2 often includes tags (e.g., His-tag) for isolation and is used in binding assays, structural studies, or probing interactions with partners like scribble or aPKC. Recent work also explores its therapeutic potential, as restoring LLGL2 function in cancers may suppress metastasis. Research on recombinant LLGL2 bridges cell biology insights with translational applications in oncology and developmental disorders.
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