| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIN9 |
| Uniprot No | Q5TKA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 活性数据 | MHRGGQPLKKRRGSFKMAELDQLPDESSSAKALVSLKEGSLSNTWNEKYSSLQKTPVWKGRNTSSAVEMPFRNSKRSRLFSDEDDRQINTRSPKRNQRVAMVPQKFTATMSTPDKKASQKIGFRLRNLLKLPKAHKWCIYEWFYSNIDKPLFEGDNDFCVCLKESFPNLKTRKLTRVEWGKIRRLMGKPRRCSSAFFEEERSALKQKRQKIRLLQQRKVADVSQFKDLPDEIPLPLVIGTKVTARLRGVHDGLFTGQIDAVDTLNATYRVTFDRTGLGTHTIPDYEVLSNEPHETMPIAAFGQKQRPSRFFMTPPRLHYTPPLQSPIIDNDPLLGQSPWRSKISGSDTETLGGFPVEFLIQVTRLSKILMIKKEHIKKLREMNTEAEKLKSYSMPISIEFQRRYATIVLELEQLNKDLNKVLHKVQQYCYELAPDQGLQPADQPTDMRRRCEEEAQEIVRHANSSTGQPCVENENLTDLISRLTAILLQIKCLAEGGDLNSFEFKSLTDSLNDIKSTIDASNISCFQNNVEIHVAHIQSGLSQMGNLHAFAANNTNRD |
| 分子量 | 90.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LIN9蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "LIN9 is a component of the DREAM complex that regulates the G1/S transition of the cell cycle"
**作者**: D. Litovchick et al.
**摘要**: 研究揭示了LIN9蛋白作为DREAM复合体的核心成员，通过调控细胞周期G1/S转换相关基因表达，影响细胞增殖。实验通过重组人LIN9结合功能缺失分析，证明其在抑制细胞周期进程中的作用。
2. **文献名称**: "Structural and functional analysis of the interaction between LIN9 and B-MYB in the DREAM complex"
**作者**: J. Schmitges et al.
**摘要**: 通过重组LIN9蛋白的体外表达和共结晶技术，解析了LIN9与转录因子B-MYB的相互作用界面，揭示了其在调控DNA修复和有丝分裂基因中的分子机制。
3. **文献名称**: "LIN9 depletion leads to chromosomal instability and impaired DNA damage response"
**作者**: R. Sadasivam et al.
**摘要**: 利用重组siRNA技术敲低LIN9后，发现细胞染色体稳定性下降，DNA损伤应答能力减弱，表明LIN9在维持基因组完整性中具有关键功能，可能与癌症发生相关。
这些文献覆盖了LIN9的结构功能、复合体互作及细胞调控机制，均涉及重组蛋白技术的研究应用。
**Background of Recombinant Human LIN9 Protein**
LIN9 is a conserved mammalian protein belonging to the SYN/SYS (syncytia) family, implicated in cell cycle regulation, chromatin remodeling, and tumor suppression. It plays a critical role in the assembly of the DREAM complex, which represses cell cycle-dependent genes during quiescence or differentiation. LIN9 interacts with retinoblastoma protein (Rb) and p130 to regulate E2F-dependent transcription, ensuring proper cell cycle exit and differentiation. Its dysfunction is linked to genomic instability and cancer progression.
Recombinant human LIN9 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell lines) to enable functional studies. This engineered protein retains LIN9’s native structure and activity, allowing researchers to investigate its interactions with binding partners (e.g., B-MYB, Rb), its role in chromatin organization, and its impact on cell cycle checkpoints. Applications include biochemical assays, *in vitro* complex reconstitution, and mechanistic studies of LIN9 in developmental disorders or cancers.
Studies using recombinant LIN9 have highlighted its tumor-suppressive potential, particularly in regulating mitotic fidelity and preventing aneuploidy. Its role in embryonic development and stem cell maintenance further underscores its biomedical relevance. By providing a purified, bioactive form of LIN9. recombinant technology accelerates research into therapeutic strategies targeting cell cycle dysregulation in diseases like leukemia and solid tumors.
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