| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIN7C |
| Uniprot No | Q9NUP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 活性数据 | MAALGEPVRLERDICRAIELLEKLQRSGEVPPQKLQALQRVLQSEFCNAVREVYEHVYETVDISSSPEVRANATAKATVAAFAASEGHSHPRVVELPKTEEGLGFNIMGGKEQNSPIYISRIIPGGIADRHGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAQGKVKLVVRYTPKVLEEMESRFEKMRSAKRRQQT |
| 分子量 | 48.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LIN7C蛋白的3篇虚拟参考文献示例(注意：文献为虚构，仅供格式参考)：
1. **文献名称**: *LIN7C interacts with PALS1 to regulate epithelial cell polarity through the CRB complex*
**作者**: Joober, R., et al.
**摘要**: 研究利用重组人LIN7C蛋白进行体外结合实验，证实其与支架蛋白PALS1的直接互作，并揭示LIN7C/PALS1复合物在上皮细胞极性形成中的关键调控作用。
2. **文献名称**: *Structural basis of the LIN7C-CRB3 interaction in membrane-associated guanylate kinase complexes*
**作者**: Lee, S., et al.
**摘要**: 通过X射线晶体学解析重组人LIN7C蛋白与CRB3的复合物结构，揭示两者结合的分子机制，为研究细胞间连接复合物的组装提供结构基础。
3. **文献名称**: *Recombinant LIN7C enhances synaptic plasticity by anchoring glutamate receptors in neurons*
**作者**: Zhang, Y., et al.
**摘要**: 研究发现重组表达的人LIN7C蛋白通过与PSD-95等突触后蛋白协同作用，稳定AMPA受体在突触的定位，从而调控神经元突触可塑性。
**备注**：若需真实文献，建议通过PubMed或Web of Science检索关键词“LIN7C recombinant”或“LIN7C protein”。实际研究中，LIN7C常与细胞极性、突触功能及癌症迁移等相关。
Recombinant human LIN7C protein is a biologically engineered form of the LIN7C (lin-7 homolog C) protein, a key component of cell polarity and signaling complexes. LIN7C belongs to the LIN7 family, which includes scaffolding proteins critical for maintaining cell polarity, synaptic transmission, and receptor localization. It contains a PDZ domain enabling interactions with transmembrane proteins like receptors, ion channels, and adhesion molecules. LIN7C partners with CASK and APBA1 to form tripartite complexes that stabilize synaptic receptors (e.g., NMDA receptors) and regulate neuronal development. Dysregulation of LIN7C is linked to neurological disorders, cancer progression, and epithelial barrier dysfunction.
The recombinant protein is typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His-tag) for purification and detection. It retains structural and functional integrity, enabling *in vitro* studies of protein interactions, cellular trafficking, and signaling pathways. Researchers employ it to explore LIN7C’s role in epithelial polarization, synaptic plasticity, and disease mechanisms, such as tumor metastasis or Alzheimer’s pathology. Its recombinant form accelerates drug discovery by serving as a target for modulating cell adhesion or receptor dynamics. Overall, recombinant LIN7C is a vital tool for decoding cell biology and developing therapeutic strategies for polarity-related disorders.
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