| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT78 |
| Uniprot No | Q8N1N4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 活性数据 | MSLSPCRAQRGFSARSACSARSRGRSRGGFSSRGGFSSRSLNSFGGCLEGSRGSTWGSGGRLGVRFGEWSGGPGLSLCPPGGIQEVTINQNLLTPLKIEIDPQFQVVRTQETQEIRTLNNQFASFIDKVRFLEQQNKVLETKWHLLQQQGLSGSQQGLEPVFEACLDQLRKQLEQLQGERGALDAELKACRDQEEEYKSKYEEEAHRRATLENDFVVLKKDVDGVFLSKMELEGKLEALREYLYFLKHLNEEELGQLQTQASDTSVVLSMDNNRYLDFSSIITEVRARYEEIARSSKAEAEALYQTKYQELQVSAQLHGDRMQETKVQISQLHQEIQRLQSQTENLKKQNASLQAAITDAEQRGELALKDAQAKVDELEAALRMAKQNLARLLCEYQELTSTKLSLDVEIATYRRLLEGEECRMSGECTSQVTISSVGGSAVMSGGVGGGLGSTCGLGSGKGSPGSCCTSIVTGGSNIILGSGKDPVLDSCSVSGSSAGSSCHTILKKTVESSLKTSITY |
| 分子量 | 83.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人KRT78蛋白的模拟参考文献示例(注：以下内容为假设性创作，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Efficient Expression and Purification of Recombinant Human Keratin 78 in E. coli*
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了人KRT78基因的重组质粒，利用大肠杆菌表达系统实现了高效可溶性表达，并通过镍柱亲和层析纯化获得高纯度蛋白，为后续功能研究提供基础。
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2. **文献名称**: *KRT78 Overexpression as a Potential Biomarker in Esophageal Squamous Cell Carcinoma*
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化及Western blot分析发现，重组人KRT78在食管鳞癌组织中异常高表达，且与肿瘤侵袭性呈正相关，提示其可能作为预后标志物及治疗靶点。
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3. **文献名称**: *Structural Characterization of Recombinant KRT78 and Its Interaction with Cytoskeletal Proteins*
**作者**: Gupta S, et al.
**摘要**: 采用圆二色光谱和X射线晶体学解析了重组KRT78的二级及三维结构，并发现其与波形蛋白(vimentin)存在特异性互作，揭示了其在维持上皮细胞骨架完整性中的作用机制。
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**注意事项**：
- 以上内容为示例，实际文献需查阅PubMed、Web of Science等平台;
- KRT78研究较少，建议扩展关键词(如"keratin 78"、"cancer"、"recombinant expression")进行检索;
- 部分相关研究可能隐含于角蛋白家族综述或疾病机制论文中。
**Background of Recombinant Human KRT78 Protein**
Keratin 78 (KRT78), a member of the keratin family, belongs to the intermediate filament proteins that provide structural support to epithelial cells. Human KRT78 is classified as a type I keratin (acidic) and typically pairs with type II keratins (basic) to form a resilient cytoskeletal network. While many keratins are well-studied in the context of epithelial integrity, KRT78 remains relatively undercharacterized compared to its counterparts. It is expressed in specific epithelial tissues, including the gastrointestinal tract and cervix, suggesting roles in maintaining tissue-specific mechanical stability.
Dysregulation of keratins, including KRT78. has been implicated in epithelial pathologies such as inflammatory disorders, cancer progression, and wound-healing defects. For instance, altered KRT78 expression has been observed in colorectal cancer, where it may influence cell migration or tumor microenvironment interactions. However, its precise biological functions and regulatory mechanisms remain unclear.
Recombinant human KRT78 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. This tool facilitates investigations into KRT78's interaction partners, post-translational modifications, and its role in cellular stress responses. Researchers also utilize recombinant KRT78 to generate antibodies for diagnostic applications or to model keratin-related diseases. By advancing access to purified KRT78. recombinant technology accelerates the exploration of its contributions to both normal physiology and disease, potentially unveiling therapeutic targets for epithelial disorders.
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