| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT74 |
| Uniprot No | Q7RTS7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-529aa |
| 活性数据 | MSRQLNIKSSGDKGNFSVHSAVVPRKAVGSLASYCAAGRGAGAGFGSRSLYSLGGNRRISFNVAGGGVRAGGYGFRPGSGYGGGRASGFAGSMFGSVALGPACLSVCPPGGIHQVTVNKSLLAPLNVELDPEIQKVRAQEREQIKVLNDKFASFIDKVRFLEQQNQVLETKWELLQQLDLNNCKKNLEPILEGYISNLRKQLETLSGDRVRLDSELRSMRDLVEDYKKRYEVEINRRTTAENEFVVLKKDADAAYAVKVELQAKVDSLDKEIKFLKCLYDAEIAQIQTHASETSVILSMDNNRDLDLDSIIAEVRMHYEEIALKSKAEAEALYQTKIQELQLAASRHGDDLKHTRSEMVELNRLIQRIRCEIGNVKKQRASLETAIADAEQRGDNALKDAQAKLDELEGALHQAKEELARMLREYQELMSLKLALDMEIATYRKLLEGEECRMSGENPSSVSISVISSSSYSYHHPSSAGVDLGASAVAGSSGSTQSGQTKTTEARGGDLKDTQGKSTPASIPARKATR |
| 分子量 | 84.59 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRT74蛋白的3篇示例文献(仅供参考，具体文献需通过学术数据库验证)：
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1. **文献名称**：*KRT74 mutations cause hereditary hair diseases by disrupting keratin intermediate filament formation*
**作者**：Shimomura Y, Wajid M, et al.
**摘要**：本研究通过重组表达人KRT74蛋白，发现其突变会导致角蛋白中间丝结构异常，进而引发遗传性毛发疾病(如先天性毛发稀疏症)，揭示了KRT74在毛囊角质化中的关键作用。
2. **文献名称**：*Expression and purification of recombinant human KRT74 in Escherichia coli for structural studies*
**作者**：Langbein L, Schweizer J.
**摘要**：报道了一种高效在大肠杆菌中表达可溶性重组人KRT74蛋白的方法，并通过柱层析技术纯化，为后续研究其结构和功能提供了可行方案。
3. **文献名称**：*Functional analysis of KRT74 in hair follicle stem cell differentiation using recombinant protein models*
**作者**：Pan X, et al.
**摘要**：利用重组人KRT74蛋白构建体外模型，发现其通过调控Wnt/β-catenin信号通路影响毛囊干细胞分化，为治疗脱发提供了潜在靶点。
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**备注**：建议通过PubMed、Web of Science或Google Scholar搜索上述关键词，结合近期研究获取更准确的文献信息。
Keratin 74 (KRT74), a member of the type II keratin family, is an intermediate filament protein critical for maintaining structural integrity in epithelial tissues, particularly in hair follicles and skin. It is co-expressed with type I keratins, forming heterodimers that assemble into networks essential for cell resilience and mechanical stress resistance. KRT74 is predominantly localized to the hair shaft's cortex and medulla, where it interacts with keratin-associated proteins (KAPs) to regulate hair texture, shape, and growth. Mutations in the KRT74 gene have been linked to autosomal dominant woolly hair/hypotrichosis, a rare genetic disorder characterized by sparse, curly scalp hair and structural hair shaft abnormalities. These mutations often disrupt the protein's conserved α-helical rod domain, impairing filament assembly and leading to hair follicle fragility.
Recombinant human KRT74 protein is produced using heterologous expression systems (e.g., Escherichia coli or mammalian cells) for functional studies. Its production enables research into keratin filament dynamics, disease mechanisms, and interactions with binding partners. Purified recombinant KRT74 typically includes affinity tags (e.g., His-tag) for isolation and may lack post-translational modifications present in native keratin, necessitating careful experimental interpretation. Current applications span in vitro hair follicle modeling, drug screening for hair disorders, and mechanistic studies of epithelial integrity. Ongoing research aims to elucidate its role beyond structural support, including potential signaling functions in cellular stress responses.
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