| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT6IRS |
| Uniprot No | Q3SY84 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523aa |
| 活性数据 | MSRQFTCKSGAAAKGGFSGCSAVLSGGSSSSFRAGSKGLSGGFGSRSLYSLGGVRSLNVASGSGKSGGYGFGRGRASGFAGSMFGSVALGPVCPTVCPPGGIHQVTVNESLLAPLNVELDPEIQKVRAQEREQIKALNNKFASFIDKVRFLEQQNQVLETKWELLQQLDLNNCKNNLEPILEGYISNLRKQLETLSGDRVRLDSELRNVQDVVEDYKKRYEEEINKRTAAENEFVLLKKDVDAAYANKVELQAKVESMDQEIKFFRCLFEAEITQIQSHISDMSVILSMDNNRNLDLDSIIDEVRTQYEEIALKSKAEAEALYQTKFQELQLAAGRHGDDLKNTKNEISELTRLIQRIRSEIENVKKQASNLETAIADAEQRGDNALKDARAKLDELEGALHQAKEELARMLREYQELMSLKLALDMEIATYRKLLESEECRMSGEFPSPVSISIISSTSGGSGYGFRPSMVSGGYVANSSNCISGVCSVRGGEGRSRGSANDYKDTLGKGSSLSAPSKKTSR |
| 分子量 | 83.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRT6IRS蛋白的参考文献示例(部分为虚构或简化，建议结合实际数据库验证)：
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1. **文献名称**: *"Expression and functional analysis of recombinant human KRT6IRS in epidermal keratinocytes"*
**作者**: Li, X., Wang, Y., & Zhang, H.
**摘要**: 本研究通过哺乳动物表达系统成功制备了重组人KRT6IRS蛋白，发现其在角蛋白细胞分化中调节细胞骨架稳定性，并验证其在先天性厚甲症(pachyonychia congenita)相关突变中的功能异常。
2. **文献名称**: *"KRT6IRS mutations disrupt keratin filament assembly: Insights from in vitro reconstitution assays"*
**作者**: Smith, J.P., et al.
**摘要**: 利用大肠杆菌表达系统纯化重组KRT6IRS蛋白，结合体外纤维组装实验，揭示了特定突变导致角蛋白中间丝结构缺陷的分子机制，为遗传性皮肤病的治疗提供靶点。
3. **文献名称**: *"Recombinant KRT6IRS promotes wound healing by enhancing epithelial migration in a 3D skin model"*
**作者**: Chen, L., et al.
**摘要**: 通过HEK293细胞表达重组KRT6IRS，并证明其通过激活整合素信号通路加速表皮细胞迁移，提示其在慢性伤口修复中的潜在应用价值。
4. **文献名称**: *"Proteomic profiling of KRT6IRS-interacting proteins in squamous cell carcinoma"*
**作者**: Gupta, R., & Kumar, S.
**摘要**: 基于重组KRT6IRS的免疫共沉淀技术，鉴定了其在鳞状细胞癌中与EGFR、β-catenin等蛋白的相互作用网络，提示其参与肿瘤侵袭的调控。
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**说明**：以上文献为示例性质，实际研究中请通过**PubMed**、**Google Scholar**或**Web of Science**检索最新论文，关键词建议：*"recombinant KRT6IRS"*、*"KRT6 keratinopathy"*、*"keratin 6 mutation"*。
The recombinant human KRT6IRS protein is a engineered variant derived from the KRT6 gene family, which encodes type II keratin intermediate filaments critical for maintaining cytoskeletal integrity in epithelial cells. KRT6 isoforms (KRT6A, KRT6B, KRT6C) are typically co-expressed with KRT16/KRT17 in stratified epithelia and are upregulated during stress, wound healing, or hyperproliferative conditions like psoriasis and cancer. The "IRS" designation likely refers to specific structural or functional modifications introduced during recombinant production, such as phosphorylation-mimetic mutations at serine residues (e.g., S32/S33) that regulate keratin network reorganization under stress. Recombinant KRT6IRS retains core domains: a central α-helical rod essential for dimerization, and variable head/tail domains influencing filament assembly. Its production in systems like *E. coli* or mammalian cells enables studies on keratinopathies, epithelial barrier dysfunction, and tumor progression. KRT6 mutants are implicated in pachyonychia congenita, emphasizing its role in nail/skin disorders. Researchers use recombinant KRT6IRS to investigate disease mechanisms, screen therapeutic compounds targeting keratin aggregation, and develop biomarkers for epithelial malignancies. Its abnormal expression in squamous cell carcinomas also positions it as a potential diagnostic or prognostic indicator.
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