| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT75 |
| Uniprot No | O95678 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-551aa |
| 活性数据 | MSRQSSITFQSGSRRGFSTTSAITPAAGRSRFSSVSVARSAAGSGGLGRISSAGASFGSRSLYNLGGAKRVSINGCGSSCRSGFGGRASNRFGVNSGFGYGGGVGGGFSGPSFPVCPPGGIQEVTVNQSLLTPLHLQIDPTIQRVRAEEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWALLQEQGSRTVRQNLEPLFDSYTSELRRQLESITTERGRLEAELRNMQDVVEDFKVRYEDEINKRTAAENEFVALKKDVDAAYMNKVELEAKVKSLPEEINFIHSVFDAELSQLQTQVGDTSVVLSMDNNRNLDLDSIIAEVKAQYEDIANRSRAEAESWYQTKYEELQVTAGRHGDDLRNTKQEISEMNRMIQRLRAEIDSVKKQCSSLQTAIADAEQRGELALKDARAKLVDLEEALQKAKQDMARLLREYQELMNIKLALDVEIATYRKLLEGEECRLSGEGVSPVNISVVTSTLSSGYGSGSSIGGGNLGLGGGSGYSFTTSGGHSLGAGLGGSGFSATSNRGLGGSGSSVKFVSTTSSSQKSYTH |
| 分子量 | 87.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人KRT75蛋白的假设性参考文献示例(基于可能研究方向构建)：
---
1. **文献名称**: *"Expression and Purification of Recombinant Human KRT75 in Escherichia coli for Structural Studies"*
**作者**: Zhang, Y., Liu, X., & Wang, H.
**摘要**: 本研究利用大肠杆菌表达系统成功表达并纯化了重组人KRT75蛋白，优化了诱导条件和纯化步骤(镍柱亲和层析)。通过圆二色谱和动态光散射分析，证实重组蛋白具有正确的二级结构和可溶性，为后续研究其与毛发角蛋白疾病的相互作用奠定基础。
---
2. **文献名称**: *"Functional Characterization of Recombinant KRT75 Mutants in Hereditary Hair Shaft Disorders"*
**作者**: Smith, J.R., Brown, T.M., & Lee, C.
**摘要**: 通过体外构建KRT75基因突变体(如p.Arg340His)，在HEK293细胞中重组表达并分析其功能。研究发现，突变导致KRT75蛋白聚集并干扰中间纤维网络形成，解释了该突变导致遗传性毛发脆弱症的分子机制。
---
3. **文献名称**: *"Recombinant KRT75 Fusion Protein Enhances Wound Healing in a 3D Skin Model"*
**作者**: Gupta, S., & Patel, R.K.
**摘要**: 将重组人KRT75与类表皮生长因子融合表达，发现其在体外3D皮肤模型中显著促进角质细胞迁移和基质重塑，暗示其在慢性伤口治疗中的潜在应用价值。
---
4. **文献名称**: *"CRISPR/Cas9-Mediated KRT75 Knockout Combined with Recombinant Protein Rescue Reveals Its Role in Chemoresistance"*
**作者**: Kim, E., Park, S., & Jung, Y.
**摘要**: 通过CRISPR敲除KRT75基因的癌细胞系中，外源添加重组KRT75蛋白可恢复细胞对化疗药物的抵抗能力，表明KRT75可能通过调控细胞骨架稳定性影响肿瘤耐药性。
---
*注：以上为基于研究领域常见方向的模拟文献，实际文献需通过PubMed、Google Scholar等平台以关键词“recombinant human KRT75”检索确认。*
Recombinant human KRT75 protein is a engineered version of the keratin 75 (KRT75) protein, a member of the type II keratin family. Keratins are intermediate filament proteins critical for maintaining structural integrity in epithelial cells, hair, nails, and skin. KRT75 is specifically expressed in the hair cuticle, inner root sheath of hair follicles, and nail beds. It plays a key role in hair shaft formation, mechanical resilience, and cellular stress response. Mutations in the KRT75 gene are linked to hair disorders like pseudofolliculitis barbae and certain forms of ectodermal dysplasia, highlighting its biological importance.
Producing recombinant KRT75 involves cloning its cDNA into expression systems (e.g., Escherichia coli, mammalian cells) to generate purified, functional protein. This approach bypasses challenges in isolating native KRT75 from tissues, enabling scalable production for research. Recombinant KRT75 retains post-translational modifications when expressed in eukaryotic systems, preserving its native structure and interactions. Its high cysteine content and disulfide-bonded domains necessitate optimized purification strategies to ensure solubility and stability.
Studies using recombinant KRT75 focus on unraveling its role in hair follicle biology, pathogenic mechanisms of keratin-related diseases, and interactions with other cytoskeletal components. It also aids in developing therapeutic agents, diagnostic tools, or protein-based treatments for keratinopathies. Additionally, it serves as an antigen for antibody production in dermatopathology research. This recombinant tool accelerates mechanistic studies and translational applications in dermatology and cell biology.
×
