| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL35 |
| Uniprot No | Q6PF15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 活性数据 | MRWVRHDAPA RRGQLRRLLE HVRLPLLAPA YFLEKVEADE LLQACGECRP LLLEARACFI LGREAGALRT RPRRFMDLAE VIVVIGGCDR KGLLKLPFAD AYHPESQRWT PLPSLPGYTR SEFAACALRN DVYVSGGHIN SHDVWMFSSH LHTWIKVASL HKGRWRHKMA VVQGQLFAVG GFDGLRRLHS VERYDPFSNT WAAAAPLPEA VSSAAVASCA GKLFVIGGAR QGGVNTDKVQ CFDPKEDRWS LRSPAPFSQR CLEAVSLEDT IYVMGGLMSK IFTYDPGTDV WGEAAVLPSP VESCGVTVCD GKVHILGGRD DRGESTDKVF TFDPSSGQVE VQPSLQRCTS SHGCVTIIQS LGR |
| 分子量 | 62.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHL35蛋白的模拟参考文献(文献为假设性示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Human KLHL35 in Ubiquitin Ligase Complex Assembly"*
**作者**: Smith J. et al. (2021)
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人KLHL35蛋白，揭示了其与Cullin-3蛋白的相互作用，证明其在泛素连接酶复合体(CRL3家族)组装中的关键作用，为研究KLHL35调控的泛素化通路提供依据。
2. **文献名称**: *"Structural Characterization of the KLHL35-Cul3 Complex by Cryo-EM"*
**作者**: Tanaka R. et al. (2019)
**摘要**: 通过冷冻电镜技术解析了重组人KLHL35与Cul3蛋白的复合物结构，发现其BTB结构域介导特异性结合，并提出KLHL35可能通过底物识别参与靶蛋白的泛素依赖性降解。
3. **文献名称**: *"KLHL35 Regulates Mitochondrial Apoptosis via Interaction with Bcl-2 Family Proteins"*
**作者**: Chen L. et al. (2020)
**摘要**: 研究利用重组人KLHL35蛋白进行体外结合实验，证实其与Bcl-2家族蛋白的相互作用，提示KLHL35可能通过调控线粒体途径凋亡参与癌症细胞的生存与死亡。
4. **文献名称**: *"A Novel Role of KLHL35 in DNA Damage Repair: Insights from Recombinant Protein Studies"*
**作者**: Wang Y. et al. (2022)
**摘要**: 通过重组KLHL35蛋白的功能实验，发现其与BRCA1的结合能力，并证明其在DNA双链断裂修复中的潜在作用，为探索KLHL35在基因组稳定性中的机制提供新方向。
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**注意**：上述文献为示例性内容，实际研究中请通过**PubMed、Web of Science或Google Scholar**等平台，以关键词“recombinant human KLHL35”、“KLHL35 function”等检索最新文献。如需真实文献支持，建议补充具体研究背景或应用场景。
Recombinant human KLHL35 protein is a genetically engineered form of the Kelch-like protein 35 (KLHL35), a member of the KLHL family characterized by conserved BTB (Broad-Complex, Tramtrack, and Bric-a-brac) and Kelch repeat domains. KLHL35 is implicated in protein-protein interactions, particularly in the assembly of E3 ubiquitin ligase complexes, which regulate substrate-specific ubiquitination and subsequent proteasomal degradation. The protein's Kelch repeats mediate substrate recognition, while its BTB domain facilitates binding to Cullin-3. a core component of ubiquitin ligase systems. Though less studied compared to other KLHL family members, KLHL35 is suggested to influence cellular processes like cytoskeletal organization, cell cycle progression, and stress responses. Dysregulation of KLHL proteins is often linked to diseases, including cancers and neurodegenerative disorders. Recombinant KLHL35 is typically produced in *E. coli* or mammalian expression systems, enabling structural and functional studies to elucidate its physiological roles, interaction networks, and potential therapeutic targets. Its applications span *in vitro* binding assays, ubiquitination studies, and disease modeling. Research on KLHL35 remains nascent, emphasizing the need for deeper exploration of its biological significance and disease associations.
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